Rabbit anti-Human GRB7 Polyclonal Antibody | anti-GRB7 antibody

GRB7, ID (GRB7, Growth factor receptor-bound protein 7, B47, Epidermal growth factor receptor GRB-7, GRB7 adapter protein) (FITC)

Cat. Num. AAA6304264

• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GRB7; Polyclonal Antibody; ID (GRB7; Growth factor receptor-bound protein 7; B47; Epidermal growth factor receptor GRB-7; GRB7 adapter protein) (FITC); GRB7 adapter protein; anti-GRB7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-GRB7 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: GRB7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 297~326 amino acids from the Central region of human GRB7.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of GRB7 Antibody (Center) in mouse brain tissue lysates (35ug/lane). GRB7 (arrow) was detected using the purified Pab.)

Related Product Information for anti-GRB7 antibody

GRB7 belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with epidermal growth factor receptor (EGFR) and ephrin receptors. The protein plays a role in the integrin signaling pathway and cell migration by binding with focal adhesion kinase (FAK).

Product Categories/Family for anti-GRB7 antibody

Antibodies; Growth Factors; Cytokines

References

Depetris, R.S., et al. Nat. Struct. Mol. Biol. 16(8):833-839(2009). Chu, P.Y., et al. J. Biol. Chem. 284(30):20215-20226(2009). Mavaddat, N., et al. Cancer Epidemiol. Biomarkers Prev. 18(1):255-259(2009). Bai, T., et al. Carcinogenesis 29(3):473-479(2008). Spuches, A.M., et al. J. Mol. Recognit. 20(4):245-252(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 71979667
• NCBI GeneID: 2886
• NCBI Accession #: NP_001025173.1
• NCBI GenBank Nucleotide #: NM_001030002.2
• UniProt Accession #: Q14451; Q53YD3; Q92568; Q96DF9; Q9Y220; B2RAV1; B3KNL0; B3KWP9; B7WP75; J3KQM4
• Molecular Weight: 59,681 Da
• NCBI Official Full Name: growth factor receptor-bound protein 7 isoform a
• NCBI Official Synonym Full Names: growth factor receptor-bound protein 7
• NCBI Official Symbol: GRB7
• NCBI Protein Information: growth factor receptor-bound protein 7; B47; GRB7 adapter protein; epidermal growth factor receptor GRB-7
• UniProt Protein Name: Growth factor receptor-bound protein 7
• Protein Family: Growth factor receptor-bound protein
• UniProt Gene Name: GRB7
• UniProt Entry Name: GRB7_HUMAN

NCBI Description

The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with epidermal growth factor receptor (EGFR) and ephrin receptors. The protein plays a role in the integrin signaling pathway and cell migration by binding with focal adhesion kinase (FAK). Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2011]

Uniprot Description

GRB7: Adapter protein that interacts with the cytoplasmic domain of numerous receptor kinases and modulates down-stream signaling. Promotes activation of down-stream protein kinases, including STAT3, AKT1, MAPK1 and/or MAPK3. Promotes activation of HRAS. Plays a role in signal transduction in response to EGF. Plays a role in the regulation of cell proliferation and cell migration. Plays a role in the assembly and stability of RNA stress granules. Binds to the 5'UTR of target mRNA molecules and represses translation of target mRNA species, when not phosphorylated. Phosphorylation impairs RNA binding and promotes stress granule disassembly during recovery after cellular stress. Homodimer. Interacts (via SH2 domain) with EGFR, ERBB2, ERBB3 (when phosphorylated), ERBB4 (when phosphorylated), EPHB1, INSR, FGFR1, PDGFRA (tyrosine phosphorylated) and PDGFRB (tyrosine phosphorylated). Interacts with SHC1. Interacts with RND1. Interacts (when tyrosine phosphorylated) with FHL2 and HAX1. Interacts (via SH2 domain) with RET and PTK2/FAK1. Interacts (when not phosphorylated) with ELAVL1. In stressed cells, but not in normal cells, part of a complex that contains at least GRB7, PTK2/FAK1, STAU1, ELAVL1 and TIA1. Interacts (via SH2 domain) with KIT (phosphorylated). Interacts (via SH2 domain) with TEK/TIE2 (tyrosine phosphorylated). Belongs to the GRB7/10/14 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: focal adhesion; cell projection; stress granule; plasma membrane; cytosol

Molecular Function: identical protein binding; protein binding; SH3/SH2 adaptor activity; RNA binding; protein kinase binding; phosphoinositide binding

Biological Process: epidermal growth factor receptor signaling pathway; stress granule assembly; positive regulation of signal transduction; negative regulation of translation; blood coagulation; leukocyte migration; positive regulation of cell migration