Rabbit anti-Human GPR77 Polyclonal Antibody | anti-GPR77 antibody

GPR77, CT (GPR77, C5L2, C5a anaphylatoxin chemotactic receptor C5L2, G-protein coupled receptor 77) (FITC)

Cat. Num. AAA6304011

• Gene Names: C5AR2; C5L2; GPF77; GPR77
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GPR77; Polyclonal Antibody; CT (GPR77; C5L2; C5a anaphylatoxin chemotactic receptor C5L2; G-protein coupled receptor 77) (FITC); G-protein coupled receptor 77; anti-GPR77 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-GPR77 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: GPR77 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 297-325 amino acids from the C-terminal region of human GPR77.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(GPR77 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the GPR77 antibody detected the GPR77 protein (arrow).)

Related Product Information for anti-GPR77 antibody

The anaphylatoxins C3a (see MIM 120700), C4a (see MIM 120810), and C5a are cationic fragments generated during the complement cascade that participate in host defense. In the case of inappropriate complement activation, anaphylatoxins may be involved in autoimmunity and sepsis. C5L2 is coexpressed with the C5a receptor, C5AR (C5R1; MIM 113995), on polymorphonuclear neutrophils and may modulate C5AR activity (Gerard et al., 2005 [PubMed 16204243]).

Product Categories/Family for anti-GPR77 antibody

Antibodies; G Protein Receptors

References

Bamberg, C.E., et al. J. Biol. Chem. 285(10):7633-7644(2010). Cui, W., et al. Mol. Immunol. 46(16):3207-3217(2009). Cui, W., et al. Mol. Immunol. 46(15):3086-3098(2009). Scola, A.M., et al. Mol. Immunol. 46(6):1149-1162(2009). Scola, A.M., et al. J. Biol. Chem. 282(6):3664-3671(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 8923873
• NCBI GeneID: 27202
• NCBI Accession #: NP_060955.1
• NCBI GenBank Nucleotide #: NM_018485.2
• UniProt Accession #: Q9P296; B2RA09
• Molecular Weight: 36,080 Da
• NCBI Official Full Name: C5a anaphylatoxin chemotactic receptor 2
• NCBI Official Synonym Full Names: complement component 5a receptor 2
• NCBI Official Symbol: C5AR2
• NCBI Official Synonym Symbols: C5L2; GPF77; GPR77
• NCBI Protein Information: C5a anaphylatoxin chemotactic receptor 2; G protein-coupled receptor 77; G protein-coupled receptor C5L2; C5a anaphylatoxin chemotactic receptor C5L2
• UniProt Protein Name: C5a anaphylatoxin chemotactic receptor 2
• UniProt Gene Name: C5AR2
• UniProt Synonym Gene Names: C5L2; GPR77
• UniProt Entry Name: C5AR2_HUMAN

NCBI Description

This gene encodes a G-protein coupled receptor 1 family member involved in the complement system of the innate immune response. Unlike classical G-protein coupled receptors, the encoded protein does not associate with intracellular G-proteins. It may instead modulate signal transduction through the beta-arrestin pathway, and may alternatively act as a decoy receptor. This gene may be involved in coronary artery disease and in the pathogenesis of sepsis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]

Uniprot Description

C5AR2: Receptor for the chemotactic and inflammatory C3a, C4a and C5a anaphylatoxin peptides and also for their dearginated forms ASP/C3adesArg, C4adesArg and C5adesArg respectively. Couples weakly to G(i)-mediated signaling pathways. Belongs to the G-protein coupled receptor 1 family.

Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: apical part of cell; integral to membrane; plasma membrane; basal plasma membrane

Molecular Function: protein binding; C5a anaphylatoxin receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; negative regulation of tumor necrosis factor production; inflammatory response; chemotaxis; positive regulation of epithelial cell proliferation