Rabbit anti-Human GPR17 Polyclonal Antibody | anti-GPR17 antibody

GPR17, ID (GPR17, Uracil nucleotide/cysteinyl leukotriene receptor, G-protein coupled receptor 17, P2Y-like receptor, R12) (FITC)

Cat. Num. AAA6303813

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GPR17; Polyclonal Antibody; ID (GPR17; Uracil nucleotide/cysteinyl leukotriene receptor; G-protein coupled receptor 17; P2Y-like receptor; R12) (FITC); R12; anti-GPR17 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-GPR17 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: GPR17 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 237~266 amino acids from the Central region of human GPR17.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of GPR17 Antibody (Center) in MDA-MB435 cell line lysates (35ug/lane). GPR17 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(GPR17 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GPR17 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(GPR17 Antibody (Center) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-GPR17 antibody

Members of the G protein coupled receptor (GPCR) superfamily contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins. The organization of the GPR17 gene differs from that of many other GPCRs in that the open reading frame is distributed on 2 exons; an additional exon contains the 5 prime untranslated region. Human GPR17 is expressed as 2.3 and 6.3 kb mRNAs exclusively in brain. The 2 transcripts appear to represent alternatively polyadenylated variants. Based on protein sequence homology and the conservation of certain key residues, GPR17 appears to be closely related to the P2Y family of GPCRs. There are two nemed isoforms.

Product Categories/Family for anti-GPR17 antibody

Antibodies; G Protein Receptors

References

Pugliese, A.M., et al. Am. J. Physiol., Cell Physiol. 297 (4), C1028-C1040 (2009). Parravicini, C., et al. BMC Bioinformatics 9, 263 (2008). Ciana, P., et al. EMBO J. 25(19):4615-4627(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 238814302
• NCBI GeneID: 2840
• NCBI Accession #: NP_001154887.1
• NCBI GenBank Nucleotide #: NM_001161415.1
• UniProt Accession #: Q13304; Q8N5S7; Q9UDZ6; Q9UE21; A8K9L0; B2R9X0
• Molecular Weight: 40,989 Da
• NCBI Official Full Name: uracil nucleotide/cysteinyl leukotriene receptor isoform a
• NCBI Official Synonym Full Names: G protein-coupled receptor 17
• NCBI Official Symbol: GPR17
• NCBI Protein Information: uracil nucleotide/cysteinyl leukotriene receptor; R12; P2Y-like receptor; UDP/CysLT receptor; G-protein coupled receptor 17
• UniProt Protein Name: Uracil nucleotide/cysteinyl leukotriene receptor
• Protein Family: Uracil nucleotide/cysteinyl leukotriene receptor
• UniProt Gene Name: GPR17
• UniProt Synonym Gene Names: UDP/CysLT receptor
• UniProt Entry Name: GPR17_HUMAN

Uniprot Description

GPR17: Dual specificity receptor for uracil nucleotides and cysteinyl leukotrienes (CysLTs). Signals through G(i) and inhibition of adenylyl cyclase. May mediate brain damage by nucleotides and CysLTs following ischemia. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral; GPCR, family 1

Chromosomal Location of Human Ortholog: 2q21

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: chemokine receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway