Rabbit anti-Human GLRX5 Polyclonal Antibody | anti-GLRX5 antibody

GLRX5, CT (GLRX5, C14orf87, Glutaredoxin-related protein 5, mitochondrial, Monothiol glutaredoxin-5) (FITC)

Cat. Num. AAA6302625

• Gene Names: GLRX5; GRX5; PRSA; FLB4739; PR01238; PRO1238; C14orf87
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GLRX5; Polyclonal Antibody; CT (GLRX5; C14orf87; Glutaredoxin-related protein 5; mitochondrial; Monothiol glutaredoxin-5) (FITC); Monothiol glutaredoxin-5; anti-GLRX5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-GLRX5 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: GLRX5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 127-157 amino acids from the C-terminal region of human GLRX5.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of GLRX5 Antibody (C-term) in Jurkat cell line lysates (35ug/lane).GLRX5 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(GLRX5 Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-GLRX5 antibody

GLRX5 is monothiol glutaredoxin involved in the biogenesis of iron-sulfur clusters. GLRX5 required for normal iron homeostasis. GLRX5 is required for normal regulation of hemoglobin synthesis by the iron-sulfur protein ACO1.

Product Categories/Family for anti-GLRX5 antibody

Antibodies; Mitochondrial Proteins

References

Bergmann, A.K., et al. Pediatr Blood Cancer 54(2):273-278(2010). Camaschella, C., et al. Blood 110(4):1353-1358(2007). Wingert, R.A., et al. Nature 436(7053):1035-1039(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 42516576
• NCBI GeneID: 51218
• NCBI Accession #: NP_057501.2
• NCBI GenBank Nucleotide #: NM_016417.2
• UniProt Accession #: Q86SX6; Q0X088; Q3YML0; Q86WY3; Q8IZ54
• Molecular Weight: 16,628 Da
• NCBI Official Full Name: glutaredoxin-related protein 5, mitochondrial
• NCBI Official Synonym Full Names: glutaredoxin 5
• NCBI Official Symbol: GLRX5
• NCBI Official Synonym Symbols: GRX5; PRSA; FLB4739; PR01238; PRO1238; C14orf87
• NCBI Protein Information: glutaredoxin-related protein 5, mitochondrial; glutaredoxin 5 homolog; monothiol glutaredoxin-5
• UniProt Protein Name: Glutaredoxin-related protein 5, mitochondrial
• Protein Family: Glutaredoxin-related protein
• UniProt Gene Name: GLRX5
• UniProt Synonym Gene Names: C14orf87
• UniProt Entry Name: GLRX5_HUMAN

NCBI Description

This gene encodes a mitochondrial protein, which is evolutionarily conserved. It is involved in the biogenesis of iron-sulfur clusters, which are required for normal iron homeostasis. Mutations in this gene are associated with autosomal recessive pyridoxine-refractory sideroblastic anemia. [provided by RefSeq, May 2010]

Uniprot Description

GLRX5: Monothiol glutaredoxin involved in the biogenesis of iron-sulfur clusters. Required for normal iron homeostasis. Required for normal regulation of hemoglobin synthesis by the iron-sulfur protein ACO1. Defects in GLRX5 are a cause of anemia sideroblastic pyridoxine-refractory autosomal recessive (PRARSA). A form of sideroblastic anemia not responsive to pyridoxine. Sideroblastic anemia is characterized by anemia of varying severity, hypochromic peripheral erythrocytes, systemic iron overload secondary to chronic ineffective erythropoiesis, and the presence of bone marrow ringed sideroblasts. Sideroblasts are characterized by iron-loaded mitochondria clustered around the nucleus. Belongs to the glutaredoxin family. Monothiol subfamily.

Protein type: Oxidoreductase

Chromosomal Location of Human Ortholog: 14q32.13

Cellular Component: mitochondrion; nucleus

Molecular Function: 2 iron, 2 sulfur cluster binding; electron carrier activity; metal ion binding; protein disulfide oxidoreductase activity

Biological Process: cell redox homeostasis; hemopoiesis

Disease: Anemia, Sideroblastic, Pyridoxine-refractory, Autosomal Recessive