Rabbit anti-Human GAL3ST4 Polyclonal Antibody | anti-GAL3ST4 antibody

GAL3ST4, ID (GAL3ST4, Galactose-3-O-sulfotransferase 4, Beta-galactose-3-O-sulfotransferase 4, Gal-beta-1,3-GalNAc 3'-sulfotransferase) (APC)

Cat. Num. AAA6301072

• Gene Names: GAL3ST4; GAL3ST-4
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GAL3ST4; Polyclonal Antibody; ID (GAL3ST4; Galactose-3-O-sulfotransferase 4; Beta-galactose-3-O-sulfotransferase 4; Gal-beta-1; 3-GalNAc 3'-sulfotransferase) (APC); 3-GalNAc 3'-sulfotransferase; anti-GAL3ST4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-GAL3ST4 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: GAL3ST4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 200-230 amino acids from the Central region of human GAL3ST4.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(GAL3ST4 Antibody (Center) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the GAL3ST4 antibody detected the GAL3ST4 protein (arrow).)

Related Product Information for anti-GAL3ST4 antibody

This gene encodes a member of the galactose-3-O-sulfotransferase protein family. The product of this gene catalyzes sulfonation by transferring a sulfate to the C-3' position of galactose residues in O-linked glycoproteins. This enzyme is highly specific for core 1 structures, with asialofetuin, Gal-beta-1,3-GalNAc and Gal-beta-1,3 (GlcNAc-beta-1,6)GalNAc being good substrates.

Product Categories/Family for anti-GAL3ST4 antibody

Antibodies; Enzymes; Sulfotransferase

References

Wan, D., et al. Proc. Natl. Acad. Sci. U.S.A. 101(44):15724-15729(2004). Chandrasekaran, E.V., et al. J. Biol. Chem. 279(11):10032-10041(2004). Seko, A., et al. J. Biol. Chem. 276(28):25697-25704(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 31542835
• NCBI GeneID: 79690
• NCBI Accession #: NP_078913.3
• NCBI GenBank Nucleotide #: NM_024637.4
• UniProt Accession #: Q96RP7; Q8N3P7; Q8WZ17; Q96E33; Q9HA78; A4D2A8; D6W5U5
• Molecular Weight: 54,166 Da
• NCBI Official Full Name: galactose-3-O-sulfotransferase 4
• NCBI Official Synonym Full Names: galactose-3-O-sulfotransferase 4
• NCBI Official Symbol: GAL3ST4
• NCBI Official Synonym Symbols: GAL3ST-4
• NCBI Protein Information: galactose-3-O-sulfotransferase 4; galbeta1-3GalNAc 3'-sulfotransferase; beta-galactose-3-O-sulfotransferase 4; beta-galactose-3-O-sulfotransferase, 4; gal-beta-1,3-GalNAc 3'-sulfotransferase
• UniProt Protein Name: Galactose-3-O-sulfotransferase 4
• Protein Family: Galactose-3-O-sulfotransferase
• UniProt Gene Name: GAL3ST4
• UniProt Synonym Gene Names: Gal3ST-4
• UniProt Entry Name: G3ST4_HUMAN

NCBI Description

This gene encodes a member of the galactose-3-O-sulfotransferase protein family. The product of this gene catalyzes sulfonation by transferring a sulfate to the C-3' position of galactose residues in O-linked glycoproteins. This enzyme is highly specific for core 1 structures, with asialofetuin, Gal-beta-1,3-GalNAc and Gal-beta-1,3 (GlcNAc-beta-1,6)GalNAc being good substrates. [provided by RefSeq, Jul 2008]

Uniprot Description

GAL3ST4: Catalyzes the transfer of sulfate to beta-1,3-linked galactose residues in O-linked glycoproteins. Good substrates include asialofetuin, Gal-beta-1,3-GalNAc and Gal-beta-1,3 (GlcNAc-beta-1,6)GalNAc. Belongs to the galactose-3-O-sulfotransferase family.

Protein type: EC 2.8.2.-; Membrane protein, integral; Transferase

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: membrane; integral to membrane

Molecular Function: galactosylceramide sulfotransferase activity; 3'-phosphoadenosine 5'-phosphosulfate binding; proteoglycan sulfotransferase activity; galactose 3-O-sulfotransferase activity

Biological Process: sulfur metabolic process; glycoprotein metabolic process; cell-cell signaling; proteoglycan biosynthetic process; oligosaccharide metabolic process