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Sheep anti-Human CCNB2 Polyclonal Antibody | anti-CCNB2 antibody

CCNB2 (Cyclin B2, G2/Mitotic-specific Cyclin-B2)

Gene Names
CCNB2; HsT17299
Reactivity
Human
Applications
Western Blot
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Synonyms
CCNB2; Polyclonal Antibody; CCNB2 (Cyclin B2; G2/Mitotic-specific Cyclin-B2); Anti -CCNB2 (Cyclin B2; anti-CCNB2 antibody
Ordering
For Research Use Only!
Host
Sheep
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human Cyclin B2. Species sequence homology: mouse (79%).
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute in PBS, 0.02% sodium azide.
Applicable Applications for anti-CCNB2 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot:1ug/ml
Immunogen
Purified, E. coli-derived recombinant human Cyclin B2 (aa1-101; Accession #O95067).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-CCNB2 antibody
Cyclin B2 (also CCNB2 and G2/mitotic-specific cyclin-B2) is a 45-46kD member of the Cyclin AB subfamily, cyclin family of proteins. It is widely expressed and associates with CDK1 providing substrate specificity to a phosphorylating complex. A phosphorCDK1:Cyclin B2 complex is associated with the Golgi apparatus, where it contributes to Golgi fragmentation during mitosis. It is also possible that Cyclin B2 can substitute for Cyclin B1 during the early stages of mitosis. Human Cyclin B2 is 398aa in length. It contains two cyclin box folds (aa201-290 and 298-383) and two substrate binding sites (aa165-254 and 264-347). Phosphorylation occurs at Ser92, Thr94, Ser99, Ser204, Ser392 and Ser398. Over aa1-101, human Cyclin B2 shares 79% aa identity with mouse Cyclin B2.
Product Categories/Family for anti-CCNB2 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
45,282 Da
NCBI Official Full Name
CCNB2
NCBI Official Synonym Full Names
cyclin B2
NCBI Official Symbol
CCNB2
NCBI Official Synonym Symbols
HsT17299
NCBI Protein Information
G2/mitotic-specific cyclin-B2; OTTHUMP00000163570
UniProt Protein Name
G2/mitotic-specific cyclin-B2
UniProt Gene Name
CCNB2
UniProt Entry Name
CCNB2_HUMAN

NCBI Description

Cyclin B2 is a member of the cyclin family, specifically the B-type cyclins. The B-type cyclins, B1 and B2, associate with p34cdc2 and are essential components of the cell cycle regulatory machinery. B1 and B2 differ in their subcellular localization. Cyclin B1 co-localizes with microtubules, whereas cyclin B2 is primarily associated with the Golgi region. Cyclin B2 also binds to transforming growth factor beta RII and thus cyclin B2/cdc2 may play a key role in transforming growth factor beta-mediated cell cycle control. [provided by RefSeq]

Uniprot Description

CCNB2: Essential for the control of the cell cycle at the G2/M (mitosis) transition. Belongs to the cyclin family. Cyclin AB subfamily.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 15q22.2

Cellular Component: microtubule cytoskeleton; nucleoplasm; centrosome; membrane; cytosol

Molecular Function: protein binding; protein kinase binding

Biological Process: mitosis; thymus development; cell division; regulation of cell cycle; in utero embryonic development; mitotic nuclear envelope disassembly; T cell homeostasis; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; G2/M transition of mitotic cell cycle; growth

Research Articles on CCNB2

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Product Notes

The CCNB2 ccnb2 (Catalog #AAA630053) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The CCNB2 (Cyclin B2, G2/Mitotic-specific Cyclin-B2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CCNB2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot:1ug/ml. Researchers should empirically determine the suitability of the CCNB2 ccnb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CCNB2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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