Rabbit anti-Human, Mouse FNBP1L Polyclonal Antibody | anti-FNBP1L antibody

FNBP1L, ID (FNBP1L, C1orf39, TOCA1, Formin-binding protein 1-like, Transducer of Cdc42-dependent actin assembly protein 1) (PE)

Cat. Num. AAA6299510

• Gene Names: FNBP1L; TOCA1; C1orf39
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FNBP1L; Polyclonal Antibody; ID (FNBP1L; C1orf39; TOCA1; Formin-binding protein 1-like; Transducer of Cdc42-dependent actin assembly protein 1) (PE); Transducer of Cdc42-dependent actin assembly protein 1; anti-FNBP1L antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-FNBP1L antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: FNBP1L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 348-377 amino acids from the Central region of human FNBP1L.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(FNBP1L Antibody (Center) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the FNBP1L antibody detected the FNBP1L protein (arrow).)

Related Product Information for anti-FNBP1L antibody

The protein encoded by this gene binds to both CDC42 and N-WASP. This protein promotes CDC42-induced actin polymerization by activating the N-WASP-WIP complex and, therefore, is involved in a pathway that links cell surface signals to the actin cytoskeleton. Alternative splicing results in multiple transcript variants encoding different isoforms.

Product Categories/Family for anti-FNBP1L antibody

Antibodies; Actin Related Proteins

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Bu, W., et al. PLoS ONE 5 (8), E12153 (2010): Bu, W., et al. J. Biol. Chem. 284(17):11622-11636(2009). Huett, A., et al. J. Immunol. 182(8):4917-4930(2009). Leung, Y., et al. Cell Host Microbe 3(1):39-47(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 256600192
• NCBI GeneID: 54874
• NCBI Accession #: NP_001157945.1; NP_001020119.1
• NCBI GenBank Nucleotide #: NM_001164473.2
• UniProt Accession #: Q5T0N5; Q5T0N6; Q6B097; Q6P653; Q6R4Q4; Q9NXG1; J3QSS4
• Molecular Weight: 69,434 Da
• NCBI Official Full Name: formin-binding protein 1-like isoform 3
• NCBI Official Synonym Full Names: formin binding protein 1 like
• NCBI Official Symbol: FNBP1L
• NCBI Official Synonym Symbols: TOCA1; C1orf39
• NCBI Protein Information: formin-binding protein 1-like
• UniProt Protein Name: Formin-binding protein 1-like
• Protein Family: Formin-binding protein
• UniProt Gene Name: FNBP1L
• UniProt Synonym Gene Names: C1orf39; TOCA1; Toca-1
• UniProt Entry Name: FBP1L_HUMAN

NCBI Description

The protein encoded by this gene binds to both CDC42 and N-WASP. This protein promotes CDC42-induced actin polymerization by activating the N-WASP-WIP complex and, therefore, is involved in a pathway that links cell surface signals to the actin cytoskeleton. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

FNBP1L: Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. May bind to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promote membrane invagination and the formation of tubules. Also promotes CDC42-induced actin polymerization by activating the WASL/N-WASP-WASPIP/WIP complex, the predominant form of WASL/N-WASP in cells. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles. Essential for autophagy of intracellular bacterial pathogens. Belongs to the FNBP1 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1p22.1

Cellular Component: cytoskeleton; cytoplasmic membrane-bound vesicle; cytoplasm; plasma membrane; cell cortex

Molecular Function: protein binding; lipid binding

Biological Process: positive regulation of filopodium formation; vesicle transport along actin filament; vesicle organization and biogenesis; membrane invagination; cilium biogenesis; autophagy; membrane budding