Rabbit cdk2 Polyclonal Antibody | anti-CDK2 antibody

cdk2, phosphorylated (Thr160) (Cyclin-dependent Kinase 2)

Cat. Num. AAA629630

• Gene Names: CDK2; p33(CDK2)
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunoprecipitation, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: cdk2; Polyclonal Antibody; phosphorylated (Thr160) (Cyclin-dependent Kinase 2); Anti -cdk2; anti-CDK2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human cdk2 at ~33kD. Detects cdk2 only when phosphorylated at threonine 160. Weakly crossreacts with cdc2 phosphorylated at Thr161. Does not crossreact with other phosphorylated cyclin-dependent kinases.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

• Applicable Applications for anti-CDK2 antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in ELISA, Flow Cytometry, Western Blot and Immunoprecipitation.; Dilution: ELISA (Peptide): 1:2000; Flow Cytometry: 1:25; Western Blot: 1:1000. Incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.; Immunoprecipitation: 1:150
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Thr160 of human cdk2.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of HeLa cell extracts enriched for different stages of the cell cycle and probed with MBS629630 or control cdk2 monoclonal antibody (lower).)

Western Blot (WB)

(Western Blot analysis of extracts from HeLa cells synchronized in G0, G1/S or Mitosis, using MBS629630.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of untreated Jurkat cells, using MBS629630 versus propidium iodide (DNA content). The boxed population indicates phospho-CDK2 (Thr160)-positive cells.)

Related Product Information for anti-CDK2 antibody

Cyclin-dependent kinase 2, p33cdk2, is an important component of the cell cycle machinery. Like p34cdc2, its kinase activity is regulated by association with a cyclin subunit, by its phosphorylation state and by association with a cdk inhibitor. Inhibitory phosphorylation occurs on Thr14 and Tyr15. Inhibition of cdk2-cyclin complexes can also be attributed to association with p27Kip1 and p21Waf1/Cip1. Activation of cdk2 complexes requires dephosphorylation of Thr14 and Tyr15 by cdc25 phosphatase and phosphorylation of Thr160, which is mediated by CAK, a complex of cdk7 and cyclin H. Cdk2/cyclin E kinase activity is important for the G1 to S transition and phosphorylates the Rb protein. In S-phase, active cdk2/cyclin A complexes predominate and phosphorylate E2F, and the active cdk2 complex persists in the nucleus through G2.

Product Categories/Family for anti-CDK2 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 16936528
• NCBI GeneID: 1017
• NCBI Accession #: NP_001789.2
• NCBI GenBank Nucleotide #: NM_001798.3
• UniProt Accession #: P24941; A8K7C6; O75100
• Molecular Weight: 33kD
• NCBI Official Full Name: cyclin-dependent kinase 2 isoform 1
• NCBI Official Synonym Full Names: cyclin-dependent kinase 2
• NCBI Official Symbol: CDK2
• NCBI Official Synonym Symbols: p33(CDK2)
• NCBI Protein Information: cyclin-dependent kinase 2; p33 protein kinase; cell devision kinase 2; cdc2-related protein kinase; cell division protein kinase 2
• UniProt Protein Name: Cyclin-dependent kinase 2
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: CDK2
• UniProt Entry Name: CDK2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported. [provided by RefSeq]

Uniprot Description

CDK2: a protein kinase of the CDK family. An important component of the cell cycle machinery. Activity of CDK2 is maximal during S phase and G2. Cdk2/cyclin E kinase activity is important for the G1 to S transition and phosphorylates the Rb protein. In S-phase, active cdk2/cyclin A complexes predominate and phosphorylate E2F, and the active cdk2 complex persists in the nucleus through G2. Part of the Rb pathway disregulated in most tumors. Target of several candidate cancer drugs. However, inhibition does not always prevent cancer cell growth, possibly due to CDK redundancy. Inhibitors: BMS-265246, BMS-265246-01, R-roscovitine (CYC200, CYC202), SU9516, R547, L868276

Protein type: EC 2.7.11.22; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Cell cycle regulation; Protein kinase, CMGC; CMGC group; CDK family; CDK/CDK1 subfamily; CDK1 subfamily

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: Cajal body; centrosome; chromosome, telomeric region; X chromosome; condensed chromosome; cytosol; nucleoplasm; Y chromosome; transcription factor complex; cytoplasm; cyclin-dependent protein kinase holoenzyme complex; nucleus; endosome

Molecular Function: protein binding; cyclin binding; cyclin-dependent protein kinase activity; metal ion binding; histone kinase activity; ATP binding

Biological Process: G1 DNA damage checkpoint; meiosis; mitosis; positive regulation of transcription, DNA-dependent; histone phosphorylation; DNA repair; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; peptidyl-serine phosphorylation; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; cell division; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; positive regulation of cell proliferation; Ras protein signal transduction; mitotic cell cycle; G2/M transition of mitotic cell cycle; blood coagulation; DNA replication; centrosome duplication; potassium ion transport; positive regulation of DNA replication initiation; G1/S transition of mitotic cell cycle