Rabbit anti-Human EIF3CL Polyclonal Antibody | anti-EIF3CL antibody

EIF3CL, NT (EIF3C, EIF3S8, Eukaryotic translation initiation factor 3 subunit C, Eukaryotic translation initiation factor 3 subunit 8, eIF3 p110) (FITC)

Cat. Num. AAA6294998

• Gene Names: EIF3C; EIF3CL; EIF3S8; eIF3-p110
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: EIF3CL; Polyclonal Antibody; NT (EIF3C; EIF3S8; Eukaryotic translation initiation factor 3 subunit C; Eukaryotic translation initiation factor 3 subunit 8; eIF3 p110) (FITC); EIF3C; eIF3 p110; anti-EIF3CL antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-EIF3CL antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:10-50; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: EIF3CL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 126-155 amino acids from the N-terminal region of human EIF3CL.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(EIF3CL Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the EIF3CL antibody detected the EIF3CL protein (arrow).)

Immunohistochemistry (IHC)

(EIF3CL Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EIF3CL Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(EIF3CL Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-EIF3CL antibody

Antibodies; Translation Initiation Factors

References

Imielinski, M., et al. Nat. Genet. 41(12):1335-1340(2009). Zhou, M., et al. Proc. Natl. Acad. Sci. U.S.A. 105(47):18139-18144(2008). Masutani, M., et al. EMBO J. 26(14):3373-3383(2007). Damoc, E., et al. Mol. Cell Proteomics 6(7):1135-1146(2007). Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 83700233
• NCBI GeneID: 8663
• NCBI Accession #: NP_001032897.1; NP_003743.1
• NCBI GenBank Nucleotide #: NM_001037808.2
• UniProt Accession #: Q99613; O00215; Q9BW98; A8K7Z0; B2RXG3; B4E1D5; H3BRV0
• Molecular Weight: 104,101 Da
• NCBI Official Full Name: eukaryotic translation initiation factor 3 subunit C isoform a
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 3 subunit C
• NCBI Official Symbol: EIF3C
• NCBI Official Synonym Symbols: EIF3CL; EIF3S8; eIF3-p110
• NCBI Protein Information: eukaryotic translation initiation factor 3 subunit C
• UniProt Protein Name: Eukaryotic translation initiation factor 3 subunit C
• Protein Family: Eukaryotic translation initiation factor 3 subunit C-like protein
• UniProt Gene Name: EIF3C
• UniProt Entry Name: EIF3C_HUMAN

Uniprot Description

EIF3C: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Identified in a HCV IRES-mediated translation complex, at least composed of EIF3C, IGF2BP1, RPS3 and HCV RNA-replicon. Belongs to the eIF-3 subunit C family.

Protein type: RNA-binding; Translation; Translation initiation

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: eukaryotic translation initiation factor 3 complex; cytosol

Molecular Function: protein binding; translation initiation factor binding; translation initiation factor activity

Biological Process: cellular protein metabolic process; translation; translational initiation; gene expression; formation of translation preinitiation complex; regulation of translational initiation