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Western Blot (WB) (EIF1AY Antibody (N-term) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the EIF1AY antibody detected the EIF1AY protein (arrow).)

Rabbit anti-Human EIF1AY Polyclonal Antibody | anti-EIF1AY antibody

EIF1AY, NT (EIF1AY, Eukaryotic translation initiation factor 1A, Y-chromosomal, Eukaryotic translation initiation factor 4C) (PE)

Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
EIF1AY; Polyclonal Antibody; NT (EIF1AY; Eukaryotic translation initiation factor 1A; Y-chromosomal; Eukaryotic translation initiation factor 4C) (PE); Eukaryotic translation initiation factor 4C; anti-EIF1AY antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-EIF1AY antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:10-50
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
EIF1AY antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human EIF1AY.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(EIF1AY Antibody (N-term) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the EIF1AY antibody detected the EIF1AY protein (arrow).)

Western Blot (WB) (EIF1AY Antibody (N-term) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the EIF1AY antibody detected the EIF1AY protein (arrow).)

Immunohistochemistry (IHC)

(EIF1AY Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EIF1AY Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (EIF1AY Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EIF1AY Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-EIF1AY antibody
This gene encodes a protein similar to eukaryotic translation initiation factor 1A (EIF1A). EIF1A is required for the binding of the 43S complex (a 40S subunit, eIF2/GTP/Met-tRNAi and eIF3) to the 5' end of capped RNA.
Product Categories/Family for anti-EIF1AY antibody
References
Serajee, F.J., et al. J. Child Neurol. 24(10):1258-1261(2009). Lim, J., et al. Cell 125(4):801-814(2006). Fortna, A., et al. PLoS Biol. 2 (7), E207 (2004): Agate, R.J., et al. Mol. Biol. Evol. 21(2):384-396(2004). Skaletsky, H., et al. Nature 423(6942):825-837(2003).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
16,442 Da
NCBI Official Full Name
eukaryotic translation initiation factor 1A, Y-chromosomal
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 1A, Y-linked
NCBI Official Symbol
EIF1AY
NCBI Protein Information
eukaryotic translation initiation factor 1A, Y-chromosomal; eIF-4C; eIF-1A Y isoform; eukaryotic translation initiation factor 4C; eukaryotic translation initiation factor 1A, Y chromosome
UniProt Protein Name
Eukaryotic translation initiation factor 1A, Y-chromosomal
UniProt Gene Name
EIF1AY
UniProt Synonym Gene Names
eIF-1A Y isoform; eIF-4C
UniProt Entry Name
IF1AY_HUMAN

NCBI Description

This gene encodes a protein similar to eukaryotic translation initiation factor 1A (EIF1A). EIF1A is required for the binding of the 43S complex (a 40S subunit, eIF2/GTP/Met-tRNAi and eIF3) to the 5' end of capped RNA. [provided by RefSeq, Jul 2008]

Uniprot Description

EIF1AY: Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits. Belongs to the eIF-1A family.

Protein type: Translation; Translation initiation

Chromosomal Location of Human Ortholog: Yq11.223

Molecular Function: protein binding; translation initiation factor activity

Biological Process: translational initiation

Research Articles on EIF1AY

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Product Notes

The EIF1AY eif1ay (Catalog #AAA6294818) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EIF1AY, NT (EIF1AY, Eukaryotic translation initiation factor 1A, Y-chromosomal, Eukaryotic translation initiation factor 4C) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EIF1AY can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:10-50 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the EIF1AY eif1ay for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EIF1AY, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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