Western Blot (WB)
(DNAJA1 Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the DNAJA1 antibody detected the DNAJA1 protein (arrow).)
Rabbit anti-Human DNAJA1 Polyclonal Antibody | anti-DNAJA1 antibody
DNAJA1, ID (DNAJA1, DNAJ2, HDJ2, HSJ2, HSPF4, DnaJ homolog subfamily A member 1, DnaJ protein homolog 2, HSDJ, Heat shock 40kD protein 4, Heat shock protein J2, Human DnaJ protein 2) (APC)
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
Western Blot (WB)
(DNAJA1 Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the DNAJA1 antibody detected the DNAJA1 protein (arrow).)
Western Blot (WB)
(DNAJA1 Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the DNAJA1 antibody detected the DNAJA1 protein (arrow).)
Immunohistochemistry (IHC)
(DNAJA1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DNAJA1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(DNAJA1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DNAJA1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Western Blot (WB)
(Western blot analysis of DNAJA1 (arrow) using rabbit polyclonal DNAJA1 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DNAJA1 gene.)
Western Blot (WB)
(Western blot analysis of DNAJA1 (arrow) using rabbit polyclonal DNAJA1 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DNAJA1 gene.)
Flow Cytometry (FC/FACS)
(DNAJA1 Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(DNAJA1 Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
Uniprot Description
DNAJA1: Co-chaperone of Hsc70. Seems to play a role in protein import into mitochondria.
Protein type: Chaperone; Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: 9p13.3
Cellular Component: membrane; mitochondrion; perinuclear region of cytoplasm; cytosol; nucleus; ubiquitin ligase complex
Molecular Function: protein binding; G-protein-coupled receptor binding; low-density lipoprotein receptor binding; chaperone binding; metal ion binding; ubiquitin protein ligase binding; unfolded protein binding; Hsp70 protein binding; ATP binding
Biological Process: negative regulation of JNK activity; protein folding; positive regulation of apoptosis; DNA damage response, detection of DNA damage; response to unfolded protein; regulation of protein transport; sperm motility; response to heat; androgen receptor signaling pathway; spermatogenesis; protein refolding; negative regulation of protein ubiquitination; negative regulation of apoptosis
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Product Notes
The DNAJA1 dnaja1 (Catalog #AAA6292598) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DNAJA1, ID (DNAJA1, DNAJ2, HDJ2, HSJ2, HSPF4, DnaJ homolog subfamily A member 1, DnaJ protein homolog 2, HSDJ, Heat shock 40kD protein 4, Heat shock protein J2, Human DnaJ protein 2) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DNAJA1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 IHC: 1:50-100 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the DNAJA1 dnaja1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNAJA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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