Rabbit anti-Human, Mouse DIS3 Polyclonal Antibody | anti-DIS3 antibody

DIS3, ID (DIS3, KIAA1008, RRP44, Exosome complex exonuclease RRP44, Protein DIS3 homolog, Ribosomal RNA-processing protein 44) (AP)

Cat. Num. AAA6292223

• Gene Names: DIS3; RRP44; dis3p; EXOSC11; KIAA1008; 2810028N01Rik
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DIS3; Polyclonal Antibody; ID (DIS3; KIAA1008; RRP44; Exosome complex exonuclease RRP44; Protein DIS3 homolog; Ribosomal RNA-processing protein 44) (AP); Ribosomal RNA-processing protein 44; anti-DIS3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-DIS3 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: DIS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 302-331 amino acids from the Central region of human DIS3.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(DIS3 Antibody (Center) western blot analysis in mouse bladder tissue lysates (15ug/lane).This demonstrates the DIS3 antibody detected DIS3 protein (arrow).)

Immunohistochemistry (IHC)

(DIS3 antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DIS3 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-DIS3 antibody

Antibodies; KIAA; orf

References

Tomecki, R., et al. EMBO J. 29(14):2342-2357(2010). Andersen, J.S., et al. Nature 433(7021):77-83(2005). Lehner, B., et al. Genome Res. 14(7):1315-1323(2004). Dunham, A., et al. Nature 428(6982):522-528(2004). Scherl, A., et al. Mol. Biol. Cell 13(11):4100-4109(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 190014625
• NCBI GeneID: 22894
• NCBI Accession #: NP_001121698.1; NP_055768.3
• NCBI GenBank Nucleotide #: NM_001128226.2
• UniProt Accession #: Q9Y2L1; Q5W0P7; Q5W0P8; Q658Z7; Q7Z481; Q8WWI2; Q9UG36; A6NI21; B2RBL2
• Molecular Weight: 109,003 Da
• NCBI Official Full Name: exosome complex exonuclease RRP44 isoform b
• NCBI Official Synonym Full Names: DIS3 exosome endoribonuclease and 3'-5' exoribonuclease
• NCBI Official Symbol: DIS3
• NCBI Official Synonym Symbols: RRP44; dis3p; EXOSC11; KIAA1008; 2810028N01Rik
• NCBI Protein Information: exosome complex exonuclease RRP44; exosome component 11; DIS3 mitotic control homolog; ribosomal RNA-processing protein 44; mitotic control protein dis3 homolog
• UniProt Protein Name: Exosome complex exonuclease RRP44
• Protein Family: DIS3-like exonuclease
• UniProt Gene Name: DIS3
• UniProt Synonym Gene Names: KIAA1008; RRP44
• UniProt Entry Name: RRP44_HUMAN

Uniprot Description

DIS3: Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. DIS3 has both 3'-5' exonuclease and endonuclease activities. Belongs to the RNR ribonuclease family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.13.-; Ribonuclease; Nucleolus; RNA processing; EC 3.1.13.-; G protein regulator, misc.; EC 3.1.26.-

Chromosomal Location of Human Ortholog: 13q22.1

Cellular Component: nucleoplasm; membrane; nucleolus; exosome (RNase complex); cytosol; nuclear exosome (RNase complex)

Molecular Function: protein binding; 3'-5'-exoribonuclease activity; RNA binding; guanyl-nucleotide exchange factor activity; endonuclease activity

Biological Process: rRNA catabolic process; gene expression; rRNA processing; mRNA catabolic process, deadenylation-dependent decay; positive regulation of GTPase activity