Dot Blot (DB)
(Dot blot analysis of anti-Phospho-eNos-S1177 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
Rabbit anti-Human eNos Polyclonal Antibody | anti-eNOS antibody
eNos, phosphorylated (Ser1177) (Nitric Oxide Synthase, Endothelial, Endothelial NOS, eNOS, EC-NOS, NOS Type III, NOSIII, Constitutive NOS, cNOS, NOS3)
Reactivity
Human
Applications
ELISA, Gel Super Shift Assay
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
eNos; Polyclonal Antibody; phosphorylated (Ser1177) (Nitric Oxide Synthase; Endothelial; Endothelial NOS; eNOS; EC-NOS; NOS Type III; NOSIII; Constitutive NOS; cNOS; NOS3); Anti -eNos; anti-eNOS antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human eNos when phosphorylated at Ser1177.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, 0.17% sodium azide.
Applicable Applications for anti-eNOS antibody
ELISA (EL/EIA), Gel Shift Assay (GS/EMSA)
Application Notes
Suitable for use in ELISA and Dot Blot.
Dilution: ELISA: 1:1,000
Dot Blot: 1:500
Dilution: ELISA: 1:1,000
Dot Blot: 1:500
Immunogen
Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser1177 of human eNos (KLH).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Dot Blot (DB)
(Dot blot analysis of anti-Phospho-eNos-S1177 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
Dot Blot (DB)
(Dot blot analysis of anti-Phospho-eNos-S1177 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of Phospho-eNos-S1177 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of Phospho-eNos-S1177 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
Related Product Information for anti-eNOS antibody
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases.
Product Categories/Family for anti-eNOS antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The eNos enos (Catalog #AAA629111) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The eNos, phosphorylated (Ser1177) (Nitric Oxide Synthase, Endothelial, Endothelial NOS, eNOS, EC-NOS, NOS Type III, NOSIII, Constitutive NOS, cNOS, NOS3) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's eNos can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Gel Shift Assay (GS/EMSA). Suitable for use in ELISA and Dot Blot. Dilution: ELISA: 1:1,000 Dot Blot: 1:500. Researchers should empirically determine the suitability of the eNos enos for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "eNos, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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