Rabbit anti-Human, Mouse DCBLD2 Polyclonal Antibody | anti-DCBLD2 antibody

DCBLD2, CT (DCBLD2, CLCP1, ESDN, Discoidin, CUB and LCCL domain-containing protein 2, CUB, LCCL and coagulation factor V/VIII-homology domains protein 1, Endothelial and smooth muscle cell-derived neuropilin-like protein) (APC)

Cat. Num. AAA6290992

• Gene Names: DCBLD2; ESDN; CLCP1
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DCBLD2; Polyclonal Antibody; CT (DCBLD2; CLCP1; ESDN; Discoidin; CUB and LCCL domain-containing protein 2; CUB; LCCL and coagulation factor V/VIII-homology domains protein 1; Endothelial and smooth muscle cell-derived neuropilin-like protein) (APC); Endothelial and smooth muscle cell-derived neuropilin-like protein; anti-DCBLD2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-DCBLD2 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: DCBLD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 537-567 amino acids from the C-terminal region of human DCBLD2.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(DCBLD2 Antibody (C-term) western blot analysis in mouse NIH-3T3,Neuro-2a cell line and mouse stomach tissue and U251,A2058,A375,Sk-Br-3,T47D cell line lysates (35ug/lane).This demonstrates the DCBLD2 antibody detected the DCBLD2 protein (arrow).)

Flow Cytometry (FC/FACS)

(DCBLD2 Antibody (C-term) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-DCBLD2 antibody

DCBLD2, otherwise known as ESDN (endothelial and smooth muscle cell-derived neuropilin-like molecule) is a novel type-I transmembrane protein with the longest cleavable secretory signal sequence among eukaryotes. It is expressed in various tissues; particularly highly expressed in cultured vascular smooth muscle cells. DCBLD2 is considered to play a role in regulation of vascular cell growth and may have a wide variety of functions in other tissues including the nervous system, like neuropilins.

Product Categories/Family for anti-DCBLD2 antibody

Antibodies; Coagulation Factors

References

Rose, J. Phd, et al. Mol. Med. (2010). In press: Guo, X., et al. J. Biol. Chem. 284(43):29376-29382(2009). Marroni, F., et al. Circ Cardiovasc Genet 2(4):322-328(2009). Kim, M., et al. Mol. Cancer Res. 6(2):222-230(2008). Sadeghi, M.M., et al. Am. J. Transplant. 7(9):2098-2105(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 54792129
• NCBI GeneID: 131566
• NCBI Accession #: NP_563615.3
• NCBI GenBank Nucleotide #: NM_080927.3
• UniProt Accession #: Q96PD2; Q8N6M4; Q8TDX2; B7WNL1; D3DN41
• Molecular Weight: 86,657 Da
• NCBI Official Full Name: discoidin, CUB and LCCL domain-containing protein 2
• NCBI Official Synonym Full Names: discoidin, CUB and LCCL domain containing 2
• NCBI Official Symbol: DCBLD2
• NCBI Official Synonym Symbols: ESDN; CLCP1
• NCBI Protein Information: discoidin, CUB and LCCL domain-containing protein 2; 1700055P21Rik; coagulation factor V/VIII-homology domains protein 1; CUB, LCCL and coagulation factor V/VIII-homology domains protein 1; endothelial and smooth muscle cell-derived neuropilin-like protei
• UniProt Protein Name: Discoidin, CUB and LCCL domain-containing protein 2
• Protein Family: Discoidin, CUB and LCCL domain-containing protein
• UniProt Gene Name: DCBLD2
• UniProt Synonym Gene Names: CLCP1; ESDN
• UniProt Entry Name: DCBD2_HUMAN

Uniprot Description

DCBLD2: a type-I transmembrane protein. May be involved in vascular remodeling and influence vascular smooth muscle cell proliferation. Contains a CUB domain and a coagulation factor V/VIII homology domain, similar to the structure of the neuropilins. Plays a role in cell motility. SEMA4B appears to be one of its ligands. Strongly expressed in nerve bundles. Highly expressed in testis, heart, skeletal muscle and also in cultured vascular smooth muscle cells. Increased in lung cancers during the process of tumor progression. A target of EGF signaling in the A431 cervical cancer cell-line. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 3q12.1|3

Cellular Component: cell surface; integral to plasma membrane

Molecular Function: protein binding

Biological Process: intracellular receptor-mediated signaling pathway; wound healing; negative regulation of cell growth