Rabbit anti-Mouse CYP5A1 Polyclonal Antibody | anti-CYP5A1 antibody

CYP5A1, ID (TBXAS1, CYP5, CYP5A1, Thromboxane-A synthase, Cytochrome P450 5A1) (Azide free) (HRP)

Cat. Num. AAA6290555

• Gene Names: TBXAS1; TS; TXS; CYP5; THAS; TXAS; CYP5A1; GHOSAL; BDPLT14
• Reactivity: Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CYP5A1; Polyclonal Antibody; ID (TBXAS1; CYP5; Thromboxane-A synthase; Cytochrome P450 5A1) (Azide free) (HRP); TBXAS1; Cytochrome P450 5A1; anti-CYP5A1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-CYP5A1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CYP5A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 234-266 amino acids from the Central region of human CYP5A1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CYP5A1 Antibody (Center) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the CYP5A1 antibody detected the CYP5A1 protein (arrow).)

Immunohistochemistry (IHC)

(CYP5A1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CYP5A1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-CYP5A1 antibody

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. However, this protein is considered a member of the cytochrome P450 superfamily on the basis of sequence similarity rather than functional similarity. This endoplasmic reticulum membrane protein catalyzes the conversion of prostglandin H2 to thromboxane A2, a potent vasoconstrictor and inducer of platelet aggregation. The enzyme plays a role in several pathophysiological processes including hemostasis, cardiovascular disease, and stroke. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-CYP5A1 antibody

Antibodies; Enzymes; Cytochrome

References

Shimada, M., et al. Hum. Genet. 128(4):433-441(2010). Wang, L.H., et al. Prostaglandins Other Lipid Mediat. 68-69, 409-422 (2002): Tazawa, R., et al. Arch. Biochem. Biophys. 334(2):349-356(1996). Baek, S.J., et al. Gene 173(2):251-256(1996). Wang, L.H., et al. Arch. Biochem. Biophys. 315(2):273-278(1994).

NCBI and Uniprot Product Information

• NCBI GI #: 195972898
• NCBI GeneID: 6916
• NCBI Accession #: NP_001052.2; NP_001124438.1
• NCBI GenBank Nucleotide #: NM_001061.4
• UniProt Accession #: P24557; O14987; Q16844; Q8IUN1; Q9GZW4; Q9HD77; Q9HD78; Q9HD79; Q9HD80; Q9HD81; Q9HD82; Q9HD83
• Molecular Weight: 60,518 Da
• NCBI Official Full Name: thromboxane-A synthase isoform 1
• NCBI Official Synonym Full Names: thromboxane A synthase 1 (platelet)
• NCBI Official Symbol: TBXAS1
• NCBI Official Synonym Symbols: TS; TXS; CYP5; THAS; TXAS; CYP5A1; GHOSAL; BDPLT14
• NCBI Protein Information: thromboxane-A synthase; TXA synthase; cytochrome P450 5A1; platelet, cytochrome P450, subfamily V; cytochrome P450, family 5, subfamily A, polypeptide 1; thromboxane A synthase 1 (platelet, cytochrome P450, family 5, subfamily A)
• UniProt Protein Name: Thromboxane-A synthase
• UniProt Gene Name: TBXAS1
• UniProt Synonym Gene Names: CYP5; CYP5A1; TXA synthase; TXS
• UniProt Entry Name: THAS_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. However, this protein is considered a member of the cytochrome P450 superfamily on the basis of sequence similarity rather than functional similarity. This endoplasmic reticulum membrane protein catalyzes the conversion of prostglandin H2 to thromboxane A2, a potent vasoconstrictor and inducer of platelet aggregation. The enzyme plays a role in several pathophysiological processes including hemostasis, cardiovascular disease, and stroke. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

Uniprot Description

TBXAS1: Defects in TBXAS1 are the cause of Ghosal hematodiaphyseal dysplasia (GHDD). GHDD is a rare autosomal recessive disorder characterized by increased bone density with predominant diaphyseal involvement and aregenerative corticosteroid-sensitive anemia. Aregenerative anemia is characterized by bone marrow failure, so that functional marrow cells are regenerated slowly or not at all. Defects in TBXAS1 are the cause of thromboxane synthetase deficiency (TBXAS1 deficiency). It is characterized by hemorrhagic diathesis. Belongs to the cytochrome P450 family.

Protein type: Lipid Metabolism - arachidonic acid; EC 5.3.99.5; Isomerase; Oxidoreductase; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 7q34-q35

Cellular Component: endoplasmic reticulum membrane; integral to membrane

Molecular Function: oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; iron ion binding; thromboxane-A synthase activity; heme binding; monooxygenase activity

Biological Process: xenobiotic metabolic process; cyclooxygenase pathway; icosanoid metabolic process; positive regulation of vasoconstriction; arachidonic acid metabolic process; cellular chloride ion homeostasis

Disease: Ghosal Hematodiaphyseal Dysplasia; Bleeding Disorder, Platelet-type, 14