Rabbit anti-Human CYP2S1 Polyclonal Antibody | anti-CYP2S1 antibody

CYP2S1, CT (CYP2S1, Cytochrome P450 2S1, CYPIIS1) (PE)

Cat. Num. AAA6290528

• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Immunofluorescence
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CYP2S1; Polyclonal Antibody; CT (CYP2S1; Cytochrome P450 2S1; CYPIIS1) (PE); CYPIIS1; anti-CYP2S1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-CYP2S1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB), Immunofluorescence (IF)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CYP2S1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 406-436 amino acids from the C-terminal region of human CYP2S1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(CYP2S1 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the CYP2S1 antibody detected the CYP2S1 protein (arrow).)

Immunohistochemistry (IHC)

(CYP2S1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CYP2S1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of CYP2S1 Antibody (C-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). )

Related Product Information for anti-CYP2S1 antibody

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. In rodents, the homologous protein has been shown to metabolize certain carcinogens; however, the specific function of the human protein has not been determined.

Product Categories/Family for anti-CYP2S1 antibody

Antibodies; Enzymes; Cytochrome

References

Bui, P.H., et al. Mol. Pharmacol. 76(5):1031-1043(2009). Saito, A., et al. J. Hum. Genet. 54(6):317-323(2009). Deb, S., et al. Expert Opin Drug Metab Toxicol 5(4):367-380(2009). Jang, Y.J., et al. Ther Drug Monit 29(3):292-298(2007). Hanzawa, Y., et al. Drug Metab. Pharmacokinet. 22(2):136-140(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 13449277
• NCBI GeneID: 29785
• NCBI Accession #: NP_085125.1
• NCBI GenBank Nucleotide #: NM_030622.6
• UniProt Accession #: Q96SQ9; Q9BZ66
• Molecular Weight: 55,817 Da
• NCBI Official Full Name: cytochrome P450 2S1
• NCBI Official Synonym Full Names: cytochrome P450, family 2, subfamily S, polypeptide 1
• NCBI Official Symbol: CYP2S1
• NCBI Protein Information: cytochrome P450 2S1; CYPIIS1; cytochrome P450, subfamily IIS, polypeptide 1; cytochrome P540, subfamily IIS, polypeptide 1; cytochrome P450 family member predicted from ESTs
• UniProt Protein Name: Cytochrome P450 2S1
• Protein Family: Cytochrome
• UniProt Gene Name: CYP2S1
• UniProt Entry Name: CP2S1_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. In rodents, the homologous protein has been shown to metabolize certain carcinogens; however, the specific function of the human protein has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

CYP2S1: Has a potential importance for extrahepatic xenobiotic metabolism. Belongs to the cytochrome P450 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Xenobiotic Metabolism - metabolism by cytochrome P450; Oxidoreductase; EC 1.14.14.1

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; endoplasmic reticulum; cytoplasm

Molecular Function: iron ion binding; heme binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; arachidonic acid epoxygenase activity; steroid hydroxylase activity; oxygen binding

Biological Process: xenobiotic metabolic process; exogenous drug catabolic process; epoxygenase P450 pathway