Rabbit anti-Human CTSD Polyclonal Antibody | anti-CTSD antibody

CTSD, CT (CTSD, CPSD, Cathepsin D, Cathepsin D light chain, Cathepsin D heavy chain) (FITC)

Cat. Num. AAA6289905

• Gene Names: CTSD; CPSD; CLN10
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: CTSD; Polyclonal Antibody; CT (CTSD; CPSD; Cathepsin D; Cathepsin D light chain; Cathepsin D heavy chain) (FITC); Cathepsin D heavy chain; anti-CTSD antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CTSD
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-CTSD antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CTSD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 383~412 amino acids from the C-terminal region of human CTSD.
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of CTSD Antibody (C-term) in A375, MDA-MB231 cell line lysates (35ug/lane). CTSD (arrow) was detected using the purified Pab.)

Related Product Information for anti-CTSD antibody

CTSD encodes a lysosomal aspartyl protease composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. This proteinase, which is a member of the peptidase C1 family, has a specificity similar to but narrower than that of pepsin A. Transcription of this gene is initiated from several sites, including one which is a start site for an estrogen-regulated transcript.

Product Categories/Family for anti-CTSD antibody

Antibodies; Cathepsin

References

Christensen, B., et al. J. Biol. Chem. 285(11):7929-7937(2010). Albayrak, O., et al. J. Gerontol. A Biol. Sci. Med. Sci. 65(3):219-224(2010). Heinrich, M., et al. EMBO J. 18(19):5252-5263(1999).

NCBI and Uniprot Product Information

• NCBI GI #: 4503143
• NCBI GeneID: 1509
• NCBI Accession #: NP_001900.1
• NCBI GenBank Nucleotide #: NM_001909.4
• UniProt Accession #: P07339; Q6IB57
• Molecular Weight: 44,552 Da
• NCBI Official Full Name: cathepsin D preproprotein
• NCBI Official Synonym Full Names: cathepsin D
• NCBI Official Symbol: CTSD
• NCBI Official Synonym Symbols: CPSD; CLN10
• NCBI Protein Information: cathepsin D; lysosomal aspartyl protease; lysosomal aspartyl peptidase; ceroid-lipofuscinosis, neuronal 10
• UniProt Protein Name: Cathepsin D
• Protein Family: Cathepsin
• UniProt Gene Name: CTSD
• UniProt Synonym Gene Names: CPSD
• UniProt Entry Name: CATD_HUMAN

NCBI Description

This gene encodes a lysosomal aspartyl protease composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. This proteinase, which is a member of the peptidase C1 family, has a specificity similar to but narrower than that of pepsin A. Transcription of this gene is initiated from several sites, including one which is a start site for an estrogen-regulated transcript. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease. [provided by RefSeq, Jul 2008]

Uniprot Description

CTSD: Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease. Consists of a light chain and a heavy chain. Belongs to the peptidase A1 family.

Protein type: Protease; Autophagy; EC 3.4.23.5; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: extracellular matrix; lysosomal lumen; extracellular space; lysosome; melanosome; extracellular region

Molecular Function: protein binding; aspartic-type endopeptidase activity

Biological Process: extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; antigen processing and presentation of exogenous peptide antigen via MHC class II; proteolysis

Disease: Ceroid Lipofuscinosis, Neuronal, 10