Rabbit anti-Human CRYBB3 Polyclonal Antibody | anti-CRYBB3 antibody

CRYBB3, CT (CRYBB3, CRYB3, Beta-crystallin B3, Beta-B3 crystallin) (Azide free) (HRP)

Cat. Num. AAA6289059

• Gene Names: CRYBB3; CRYB3; CATCN2; CTRCT22
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CRYBB3; Polyclonal Antibody; CT (CRYBB3; CRYB3; Beta-crystallin B3; Beta-B3 crystallin) (Azide free) (HRP); Beta-B3 crystallin; anti-CRYBB3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-CRYBB3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CRYBB3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 178-207 amino acids from the C-terminal region of human CRYBB3.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CRYBB3 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CRYBB3 antibody detected the CRYBB3 protein (arrow).)

Product Categories/Family for anti-CRYBB3 antibody

Antibodies; Crystallins

References

Muller, C., et al. Anim. Genet. 39(1):87-88(2008). Wu, C., et al. Proteomics 7(11):1775-1785(2007). Riazuddin, S.A., et al. Invest. Ophthalmol. Vis. Sci. 46(6):2100-2106(2005). Collins, J.E., et al. Genome Biol. 5 (10), R84 (2004): MacCoss, M.J., et al. Proc. Natl. Acad. Sci. U.S.A. 99(12):7900-7905(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 4758074
• NCBI GeneID: 1417
• NCBI Accession #: NP_004067.1
• NCBI GenBank Nucleotide #: NM_004076.4
• UniProt Accession #: P26998; Q3B7S9; Q3T1B7; Q6ISK6; Q92965; Q9UH09
• Molecular Weight: 24,252 Da
• NCBI Official Full Name: beta-crystallin B3
• NCBI Official Synonym Full Names: crystallin beta B3
• NCBI Official Symbol: CRYBB3
• NCBI Official Synonym Symbols: CRYB3; CATCN2; CTRCT22
• NCBI Protein Information: beta-crystallin B3
• UniProt Protein Name: Beta-crystallin B3
• Protein Family: Beta-crystallin
• UniProt Gene Name: CRYBB3
• UniProt Synonym Gene Names: CRYB3
• UniProt Entry Name: CRBB3_HUMAN

NCBI Description

Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Beta-crystallins, the most heterogeneous, differ by the presence of the C-terminal extension (present in the basic group, none in the acidic group). Beta-crystallins form aggregates of different sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. This gene, a beta basic group member, is part of a gene cluster with beta-A4, beta-B1, and beta-B2. Mutations in this gene result in cataract congenital nuclear autosomal recessive type 2. [provided by RefSeq, Feb 2013]

Uniprot Description

CRYBB3: a major structural protein of the eye lens. A member of the beta/gamma-crystallin family.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 22q11.23

Molecular Function: structural constituent of eye lens

Biological Process: visual perception

Disease: Cataract 22, Multiple Types