Rabbit anti-Rat Vesicular Monoamine Transporter 1 Polyclonal Antibody | anti-SLC18A2 antibody

Vesicular Monoamine Transporter 1 (VMAT1)

Cat. Num. AAA628826

• Gene Names: SLC18A2; SVAT; SVMT; VAT2; VMAT2
• Reactivity: Rat
• Applications: ELISA, Western Blot
• Purity: Serum; Serum
• Synonyms: Vesicular Monoamine Transporter 1; Polyclonal Antibody; Vesicular Monoamine Transporter 1 (VMAT1); Anti -Vesicular Monoamine Transporter 1 (VMAT1); anti-SLC18A2 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes rat VMAT1. Does not recognize VMAT2 in transfected cells. The C-terminal rat VMAT1 sequence does not show any appreciable homology with hVMAT1.
• Purity/Purification: Serum; Serum
• Form/Format: Supplied as a lyophilized powder from PBS, 0.05% sodium azide. Reconstitute with 100ul sterile PBS.

• Applicable Applications for anti-SLC18A2 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1:1000-1:5000 (ECL); ELISA: 1:10,000-50,000. Control peptide can be used to coat ELISA plates at 1ug/ml.
• Immunogen: Synthetic peptide corresponding to 12aa near the C-terminus, cytoplasmic domain of rat VMAT1 (KLH). (Accession #Q01818)
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-SLC18A2 antibody

The regulated exocytotic release of neurotransmitters in response to neural activity requires storage within intracellular vesicles. In the nervous system, these vesicles are the synaptic vesicles that are derived from the endosomal compartment, whereas in endocrine cells larger secretory granules, such as the chromaffin granules of adrenal medulla, are derived from the trans golgi networks. For classical transmitters that are synthesized in the cytoplasm or appear there after removal form the synapses by plasma membrane reuptake, storage depends upon the active transport into the vesicles. Several distinct transport activities have been identified for monoamines, acetylcholine, glutamate, GABA and glycine. Vesicular monoamine transporters (VMATs) catalyze transport and storage of monoamines, serotonin, dopamine, norepinephrine, epinephrine, and histamine. The driving force utilized by the VMAT is the H+ electrochemical gradient generated by a vacuolar ATP-dependent H+ pump (V-ATPase) located on vesicular plasma membrane. VMAT is inhibited by a wide variety of compounds including reserpine and tetrabenazine.

Product Categories/Family for anti-SLC18A2 antibody

Antibodies; Abs to Transport Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 296189
• NCBI GeneID: 6571
• UniProt Accession #: Q05940; Q15876; Q5VW49; Q9H3P6; B2RC96; D3DRC4
• Molecular Weight: 55,713 Da
• NCBI Official Full Name: vesicular monoamine transporter
• NCBI Official Synonym Full Names: solute carrier family 18 (vesicular monoamine transporter), member 2
• NCBI Official Symbol: SLC18A2
• NCBI Official Synonym Symbols: SVAT; SVMT; VAT2; VMAT2
• NCBI Protein Information: synaptic vesicular amine transporter; monoamine transporter; vesicular amine transporter 2; vesicle monoamine/H+ antiporter; solute carrier family 18 member 2; vesicle monoamine transporter type 2; monoamine neurotransmitter transporter; solute carrier family 18 (vesicular monoamine), member 2
• UniProt Protein Name: Synaptic vesicular amine transporter
• Protein Family: Synaptic vesicular amine transporter
• UniProt Gene Name: SLC18A2
• UniProt Synonym Gene Names: SVMT; VMAT2; VAT2
• UniProt Entry Name: VMAT2_HUMAN

NCBI Description

The vesicular monoamine transporter acts to accumulate cytosolic monoamines into synaptic vesicles, using the proton gradient maintained across the synaptic vesicular membrane. Its proper function is essential to the correct activity of the monoaminergic systems that have been implicated in several human neuropsychiatric disorders. The transporter is a site of action of important drugs, including reserpine and tetrabenazine (summary by Peter et al., 1993 [PubMed 7905859]). See also SLC18A1 (MIM 193002).[supplied by OMIM, Jan 2011]

Uniprot Description

SLC18A2: Involved in the ATP-dependent vesicular transport of biogenic amine neurotransmitters. Pumps cytosolic monoamines including dopamine, norepinephrine, serotonin, and histamine into synaptic vesicles. Requisite for vesicular amine storage prior to secretion via exocytosis. Belongs to the major facilitator superfamily. Vesicular transporter family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter; Transporter, SLC family

Chromosomal Location of Human Ortholog: 10q25

Cellular Component: synaptic vesicle; cell soma; membrane; synaptic vesicle membrane; integral to plasma membrane; plasma membrane; terminal button

Molecular Function: serotonin transmembrane transporter activity; enzyme binding; amine transmembrane transporter activity; heat shock protein binding; monoamine transmembrane transporter activity; drug binding

Biological Process: dopamine transport; amine transport; neurotransmitter secretion; death; monoamine transport; response to amphetamine; locomotory behavior; response to herbicide; glucose homeostasis; sequestering of neurotransmitter; endocytic recycling; response to cocaine; post-embryonic development; response to starvation; synaptic transmission; response to zinc ion; insulin secretion; serotonin transport; transmembrane transport; negative regulation of neurotransmitter transport; response to corticosterone stimulus; aging