Rabbit anti-Human CPA6 Polyclonal Antibody | anti-CPA6 antibody

CPA6, ID (CPA6, CPAH, Carboxypeptidase A6, Carboxypeptidase B) (Biotin)

Cat. Num. AAA6288232

• Gene Names: CPA6; CPAH; ETL5; FEB11
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CPA6; Polyclonal Antibody; ID (CPA6; CPAH; Carboxypeptidase A6; Carboxypeptidase B) (Biotin); Carboxypeptidase B; anti-CPA6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-CPA6 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CPA6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 292-323 amino acids from the Central region of human CPA6.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CPA6 Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the CPA6 antibody detected the CPA6 protein (arrow).)

Related Product Information for anti-CPA6 antibody

The protein encoded by this gene belongs to the family of carboxypeptidases, which catalyze the release of C-terminal amino acid, and have functions ranging from digestion of food to selective biosynthesis of neuroendocrine peptides. Polymorphic variants and a reciprocal translocation t(6;8)(q26;q13) involving this gene, have been associated with Duane retraction syndrome.

Product Categories/Family for anti-CPA6 antibody

Antibodies; Enzymes; Carboxypeptidase

References

Bailey, S.D., et al. Diabetes Care (2010). In press: Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Sharif, S.A., et al. Arthritis Rheum. 60(10):2902-2912(2009). Lyons, P.J., et al. J. Biol. Chem. 283(11):7054-7063(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 188536067
• NCBI GeneID: 57094
• NCBI Accession #: NP_065094.3
• NCBI GenBank Nucleotide #: NM_020361.4
• UniProt Accession #: Q8N4T0; Q8NEX8; Q8TDE8; Q9NRI9
• Molecular Weight: 51,008 Da
• NCBI Official Full Name: carboxypeptidase A6 preproprotein
• NCBI Official Synonym Full Names: carboxypeptidase A6
• NCBI Official Symbol: CPA6
• NCBI Official Synonym Symbols: CPAH; ETL5; FEB11
• NCBI Protein Information: carboxypeptidase A6; carboxypeptidase B
• UniProt Protein Name: Carboxypeptidase A6
• Protein Family: Carboxypeptidase
• UniProt Gene Name: CPA6
• UniProt Synonym Gene Names: CPAH
• UniProt Entry Name: CBPA6_HUMAN

NCBI Description

The protein encoded by this gene belongs to the family of carboxypeptidases, which catalyze the release of C-terminal amino acid, and have functions ranging from digestion of food to selective biosynthesis of neuroendocrine peptides. Polymorphic variants and a reciprocal translocation t(6;8)(q26;q13) involving this gene, have been associated with Duane retraction syndrome.[provided by RefSeq, Sep 2010]

Uniprot Description

CPA6: May be involved in the proteolytic inactivation of enkephalins and neurotensin in some brain areas. May convert inactive angiotensin I into the biologically active angiotensin II. A chromosomal aberration involving CPA6 was found in a patient with Duane retraction syndrome. Translocation t(6;8)(q26;q13). Defects in CPA6 are the cause of epilepsy, familial temporal lobe, type 5 (ETL5). ETL5 is a focal form of epilepsy characterized by recurrent seizures that arise from foci within the temporal lobe. Seizures are usually accompanied by sensory symptoms, most often auditory in nature. Defects in CPA6 are the cause of familial febrile convulsions type 11 (FEB11). FEB11 consists of seizures associated with febrile episodes in childhood without any evidence of intracranial infection or defined pathologic or traumatic cause. It is a common condition, affecting 2-5% of children aged 3 months to 5 years. The majority are simple febrile seizures (generally defined as generalized onset, single seizures with a duration of less than 30 minutes). Complex febrile seizures are characterized by focal onset, duration greater than 30 minutes, and/or more than one seizure in a 24 hour period. The likelihood of developing epilepsy following simple febrile seizures is low. Complex febrile seizures are associated with a moderately increased incidence of epilepsy. Belongs to the peptidase M14 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; EC 3.4.17.1; Secreted; Protease

Chromosomal Location of Human Ortholog: 8q13.2

Cellular Component: proteinaceous extracellular matrix

Molecular Function: zinc ion binding; metallocarboxypeptidase activity

Biological Process: proteolysis

Disease: Epilepsy, Familial Temporal Lobe, 5; Febrile Seizures, Familial, 11