Rabbit anti-Human Claudin 16 Polyclonal Antibody | anti-CLDN16 antibody
Anti-Claudin 16 (Paracellin 1, CLDN16)
Gene Names
CLDN16; HOMG3; PCLN1
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Serum
Synonyms
Claudin 16; Polyclonal Antibody; Anti-Claudin 16 (Paracellin 1; CLDN16); Paracellin 1; CLDN16; anti-CLDN16 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human Claudin 16 (Paracellin 1) is 70% conserved in bovine.
Purity/Purification
Serum
Form/Format
Supplied as a liquid, neat serum, 40% glycerol.
Applicable Applications for anti-CLDN16 antibody
ELISA; Western Blot
Application Notes
Recommended Dilutions: Western Blot: 1:1-:1:3K) using ECL technique.
ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity pure. Control peptide can be used to coat ELISA plates at 1ug/ml.
ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity pure. Control peptide can be used to coat ELISA plates at 1ug/ml.
Immunogen
A 17 aa peptide sequence within the cytoplasmic, C-terminus of human Claudin 16 (Paracellin 1)
Source Information
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Shipping Temperature: Blue Ice
Shipping Temperature: Blue Ice
Related Product Information for anti-CLDN16 antibody
Magnesium as a cofactor is required in many cellular activities. Mg+2 reabsorption in the kidney is mediated primarily be a poorly understood paracellular pathway (passage of solutes between the cells) in the thick ascending limb of Henle (TAL). Tight junctions constitute the barrier to paracellular conductance. Familial hypomagnesaemia with hypercalciuria and nephrocalcinosis (FHHNC, MIM 248250) is a complex renal tubular disorder characterized by hypomagnesaemia, hypercalciuria, advanced nephrocalcinosis, hyposthenuria and progressive renal failure. The mode of inheritance is autosomal recessive. A primary defect in the reabsorption of magnesium in the TAL has been proposed to be essential in FHHNC pathophysiology. Recently, mutations in the gene paracellin-1 (Claudin 16) have been identified as the underlying genetic defect in FHHNC. Null mutation of Claudin-16 has been shown to produce chronic interstitial nephritis in cattle. PCLN-1 gene codes for a protein of 305 aa (chromosome 3q27), with four TM domains and intracellular NH2- and COOH-termini. Claudin-16 is only expressed in tight junction of TAL implicated in Mg+2-reabsorption.
Product Categories/Family for anti-CLDN16 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
33,836 Da
NCBI Official Full Name
claudin-16
NCBI Official Synonym Full Names
claudin 16
NCBI Official Symbol
CLDN16
NCBI Official Synonym Symbols
HOMG3; PCLN1
NCBI Protein Information
claudin-16; PCLN-1; paracellin-1; OTTHUMP00000209499; hypomagnesemia 3, with hypercalciuria and nephrocalcinosis
UniProt Protein Name
Claudin-16
Protein Family
UniProt Gene Name
CLDN16
UniProt Synonym Gene Names
PCLN1
UniProt Entry Name
CLD16_HUMAN
Similar Products
Product Notes
The CLDN16 cldn16 (Catalog #AAA628812) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Claudin 16 (Paracellin 1, CLDN16) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Claudin 16 can be used in a range of immunoassay formats including, but not limited to, ELISA; Western Blot. Recommended Dilutions: Western Blot: 1:1-:1:3K) using ECL technique. ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity pure. Control peptide can be used to coat ELISA plates at 1ug/ml. Researchers should empirically determine the suitability of the CLDN16 cldn16 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Claudin 16, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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