Rabbit anti-Human CMKOR1 Polyclonal Antibody | anti-CMKOR1 antibody

CMKOR1, CT (CXCR7, CMKOR1, GPR159, RDC1, C-X-C chemokine receptor type 7, Chemokine orphan receptor 1, G-protein coupled receptor 159, G-protein coupled receptor RDC1 homolog) (PE)

Cat. Num. AAA6287052

• Gene Names: CXCR7; RDC1; RDC-1; CMKOR1; CXC-R7; CXCR-7; GPR159
• Reactivity: Human
• Applications: Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: CMKOR1; Polyclonal Antibody; CT (CXCR7; GPR159; RDC1; C-X-C chemokine receptor type 7; Chemokine orphan receptor 1; G-protein coupled receptor 159; G-protein coupled receptor RDC1 homolog) (PE); CXCR7; G-protein coupled receptor RDC1 homolog; anti-CMKOR1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CMKOR1.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-CMKOR1 antibody: Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 333-362 in the C-terminal region of human CMKOR1, Swiss/UniProt Accession: P25106
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in U251 cell line lysates (35ug/lane) usingMBS6008553. This demonstrates that MBS6008553 detected the CMKOR1 protein (arrow).)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of U251 cells using MBS6008553 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CMKOR1 antibody

CXCR7 encodes a member of the G-protein coupled receptor family. Although this protein was earlier thought to be a receptor for vasoactive intestinal peptide (VIP), it is now considered to be an orphan receptor, in that its endogenous ligand has not been identified. The protein is also a coreceptor for human immunodeficiency viruses (HIV). Translocations involving this gene and HMGA2 on chromosome 12 have been observed in lipomas. [provided by RefSeq].

Product Categories/Family for anti-CMKOR1 antibody

Antibodies; G Protein Receptors

References

1. Berahovich, R.D., et al. J. Immunol. 185(9):5130-5139(2010). 2. Watanabe, K., et al. Arthritis Rheum. 62(11):3211-3220(2010). 3. Hattermann, K., et al. Cancer Res. 70(8):3299-3308(2010). 4. Miekus, K., et al. Folia Histochem. Cytobiol. 48(1):104-111(2010). 5. Zheng, K., et al. J. Exp. Clin. Cancer Res. 29, 31 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 31083344
• NCBI GeneID: 57007
• NCBI Accession #: NP_064707.1
• NCBI GenBank Nucleotide #: NM_020311.2
• UniProt Accession #: P25106; Q53RV4; Q8NE10; Q92938; Q92986; A8K6J4
• Molecular Weight: 41,493 Da
• NCBI Official Full Name: C-X-C chemokine receptor type 7
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) receptor 7
• NCBI Official Symbol: CXCR7
• NCBI Official Synonym Symbols: RDC1; RDC-1; CMKOR1; CXC-R7; CXCR-7; GPR159
• NCBI Protein Information: C-X-C chemokine receptor type 7; G protein-coupled receptor; chemokine orphan receptor 1; G-protein coupled receptor 159; G-protein coupled receptor RDC1 homolog
• UniProt Protein Name: C-X-C chemokine receptor type 7
• UniProt Gene Name: CXCR7
• UniProt Synonym Gene Names: CMKOR1; GPR159; RDC1; CXC-R7; CXCR-7; RDC-1
• UniProt Entry Name: CXCR7_HUMAN

NCBI Description

This gene encodes a member of the G-protein coupled receptor family. Although this protein was earlier thought to be a receptor for vasoactive intestinal peptide (VIP), it is now considered to be an orphan receptor, in that its endogenous ligand has not been identified. The protein is also a coreceptor for human immunodeficiency viruses (HIV). Translocations involving this gene and HMGA2 on chromosome 12 have been observed in lipomas. [provided by RefSeq, Jul 2008]

Uniprot Description

CMKOR1: Receptor for chemokines CXCL12/SDF1 and CXCL11. Does not elicit classical chemokine receptor signaling; chemokine binding does not activate G-protein-mediated signal transduction but instead induces beta-arrestin recruitment, leading to ligand internalization and activation of MAPK signaling pathway. Acts as a scavenger for CXCL12/SDF1 and, to a lesser extent, for CXCL11. Required for regulation of CXCR4 protein levels in migrating interneurons, thereby adapting their chemokine responsiveness. In glioma cells, transduces signals via MEK/ERK pathway, mediating resistance to apoptosis. Promotes cell growth and survival. Not involved in cell migration, adhesion or proliferation of normal hematopoietic progenitors but activated by CXCL11 in malignant hemapoietic cells, leading to phosphorylation of ERK1/2 (MAPK3/MAPK1) and enhanced cell adhesion and migration. Plays a regulatory role in CXCR4-mediated activation of cell surface integrins by CXCL12. Required for heart valve development. Acts as coreceptor with CXCR4 for a restricted number of HIV isolates. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: recycling endosome; cell surface; perinuclear region of cytoplasm; early endosome; plasma membrane; integral to membrane; coated pit; endosome

Molecular Function: C-X-C chemokine binding; protein binding; coreceptor activity; scavenger receptor activity; C-X-C chemokine receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; viral reproduction; receptor internalization; angiogenesis; cell adhesion; vasculogenesis; chemotaxis