Rabbit anti-Human CENPE Polyclonal Antibody | anti-CENPE antibody

CENPE, CT (CENPE, Centromere-associated protein E, Centromere protein E, Kinesin-related protein CENPE) (PE)

Cat. Num. AAA6285094

• Gene Names: CENPE; KIF10; CENP-E; MCPH13; PPP1R61
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CENPE; Polyclonal Antibody; CT (CENPE; Centromere-associated protein E; Centromere protein E; Kinesin-related protein CENPE) (PE); Kinesin-related protein CENPE; anti-CENPE antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Sequence Length: 2701

• Applicable Applications for anti-CENPE antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CENPE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2572-2602 amino acids from the C-terminal region of human CENPE.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(CENPE Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the CENPE antibody detected the CENPE protein (arrow).)

Related Product Information for anti-CENPE antibody

Centrosome-associated protein E is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. CENPE is proposed to be one of the motors responsible for mammalian chromosome movement and/or spindle elongation.

Product Categories/Family for anti-CENPE antibody

Antibodies; Centromeres

References

Kim, Y., et al. Cell 142(3):444-455(2010). Maia, A.F., et al. Chromosoma 119(4):405-413(2010). Kalsi, G., et al. Hum. Mol. Genet. 19(12):2497-2506(2010). Maffini, S., et al. Curr. Biol. 19(18):1566-1572(2009). Liu, Z., et al. J. Exp. Clin. Cancer Res. 28, 156 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 71061468
• NCBI GeneID: 1062
• NCBI Accession #: NP_001804
• NCBI GenBank Nucleotide #: NP_001804.2
• UniProt Accession #: Q02224
• NCBI Official Full Name: centromere-associated protein E isoform 1
• NCBI Official Synonym Full Names: centromere protein E
• NCBI Official Symbol: CENPE
• NCBI Official Synonym Symbols: KIF10; CENP-E; MCPH13; PPP1R61
• NCBI Protein Information: centromere-associated protein E
• UniProt Protein Name: Centromere-associated protein E
• Protein Family: Centromere-associated protein
• UniProt Gene Name: CENPE
• UniProt Synonym Gene Names: CENP-E
• UniProt Entry Name: CENPE_HUMAN

NCBI Description

Centrosome-associated protein E (CENPE) is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. This protein is required for stable spindle microtubule capture at kinetochores which is a necessary step in chromosome alignment during prometaphase. This protein also couples chromosome position to microtubule depolymerizing activity. Alternative splicing results in multiple transcript variants encoding distinct protein isoforms. [provided by RefSeq, Nov 2014]

Uniprot Description

CENPE: Essential for the maintenance of chromosomal stability through efficient stabilization of microtubule capture at kinetochores. Plays a key role in the movement of chromosomes toward the metaphase plate during mitosis. Is a slow plus end- directed motor whose activity is essential for metaphase chromosome alignment. Couples chromosome position to microtubule depolymerizing activity. The highly processive microtubule- dependent motor activity of CENPE serves to power chromosome congression and provides a flexible, motile tether linking kinetochores to dynamic spindle microtubules. Necessary for the mitotic checkpoint signal at individual kinetochores to prevent aneuploidy due to single chromosome loss. Required for the efficient recruitment of BUBR1, MAD1 and MAD2 to attached and newly unattached kinetochores. Stimulates mammalian BUBR1 kinase activity. Accumulates just before mitosis at the G2 phase of the cell cycle. Belongs to the kinesin-like protein family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motor; Microtubule-binding

Chromosomal Location of Human Ortholog: 4q24-q25

Cellular Component: kinetochore; microtubule cytoskeleton; kinesin complex; microtubule; membrane; cytoplasm; outer kinetochore of condensed chromosome; chromosome; midbody; cytosol; nucleus; chromosome, pericentric region

Molecular Function: kinetochore binding; protein binding; microtubule binding; ATPase activity; microtubule motor activity; ATP binding

Biological Process: kinetochore assembly; metabolic process; multicellular organismal development; regulation of mitotic metaphase/anaphase transition; antigen processing and presentation of exogenous peptide antigen via MHC class II; mitotic chromosome movement towards spindle pole; mitotic metaphase plate congression; microtubule-based movement; chromosome segregation; attachment of spindle microtubules to kinetochore during mitosis; cell division; positive regulation of protein kinase activity; mitotic cell cycle; blood coagulation

Disease: Microcephaly 13, Primary, Autosomal Recessive