Western Blot (WB)
(Western blot analysis of CDCA3 antibody (N-term) in mouse spleen tissue lysates(35ug/lane). CDCA3 (arrow) was detected using the purified Pab.)
Rabbit anti-Human, Mouse CDCA3 Polyclonal Antibody | anti-CDCA3 antibody
CDCA3, NT (CDCA3, C8, GRCC8, TOME1, Cell division cycle-associated protein 3, Gene-rich cluster protein C8, Trigger of mitotic entry protein 1) (MaxLight 750)
Gene Names
CDCA3; GRCC8; TOME-1
Reactivity
Human, Mouse
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Synonyms
CDCA3; Polyclonal Antibody; NT (CDCA3; C8; GRCC8; TOME1; Cell division cycle-associated protein 3; Gene-rich cluster protein C8; Trigger of mitotic entry protein 1) (MaxLight 750); Trigger of mitotic entry protein 1; anti-CDCA3 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.
Applicable Applications for anti-CDCA3 antibody
Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:50-100
Applications are based on unconjugated antibody.
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:50-100
Applications are based on unconjugated antibody.
Immunogen
CDCA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 48~77 amino acids from the N-terminal region of human CDCA3.
Conjugate
MaxLight750
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western blot analysis of CDCA3 antibody (N-term) in mouse spleen tissue lysates(35ug/lane). CDCA3 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of CDCA3 antibody (N-term) in mouse spleen tissue lysates(35ug/lane). CDCA3 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with CDCA3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with CDCA3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(CDCA3 Antibody (N-term) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(CDCA3 Antibody (N-term) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-CDCA3 antibody
CDCA3 is a F-box-like protein which is required for entry into mitosis. It acts by participating to E3 ligase complexes that mediate the ubiquitination and degradation of WEE1 kinase at G2/M phase.
Product Categories/Family for anti-CDCA3 antibody
References
Daub H., Mol. Cell 31:438-448(2008).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,998 Da
NCBI Official Full Name
cell division cycle-associated protein 3
NCBI Official Synonym Full Names
cell division cycle associated 3
NCBI Official Symbol
CDCA3
NCBI Official Synonym Symbols
GRCC8; TOME-1
NCBI Protein Information
cell division cycle-associated protein 3; gene rich cluster, C8; trigger of mitotic entry 1; gene-rich cluster protein C8; trigger of mitotic entry protein 1
UniProt Protein Name
Cell division cycle-associated protein 3
Protein Family
UniProt Gene Name
CDCA3
UniProt Synonym Gene Names
C8; GRCC8; TOME1; TOME-1
UniProt Entry Name
CDCA3_HUMAN
Uniprot Description
CDCA3: F-box-like protein which is required for entry into mitosis. Acts by participating in E3 ligase complexes that mediate the ubiquitination and degradation of WEE1 kinase at G2/M phase.
Protein type: Cell cycle regulation
Chromosomal Location of Human Ortholog: 12p13
Cellular Component: cytosol
Biological Process: mitosis; cell division; protein ubiquitination
Research Articles on CDCA3
Similar Products
Product Notes
The CDCA3 cdca3 (Catalog #AAA6284015) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CDCA3, NT (CDCA3, C8, GRCC8, TOME1, Cell division cycle-associated protein 3, Gene-rich cluster protein C8, Trigger of mitotic entry protein 1) (MaxLight 750) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CDCA3 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 IHC: 1:50-100 WB: 1:50-100 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CDCA3 cdca3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDCA3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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