Western Blot (WB)
(Western blot analysis of CD31 antibody (C-term) in HL60 cell line lysates (35ug/lane). CD31 (arrow) was detected using the purified Pab.)
Rabbit anti-Human CD31 Polyclonal Antibody | anti-CD31 antibody
CD31, CT (PECAM1, Platelet endothelial cell adhesion molecule, EndoCAM, GPIIA', PECA1, CD31) (Biotin)
ELISA: 1:1000
IHC: 1:10-50
WB: 1:50-100
Applications are based on unconjugated antibody.
Western Blot (WB)
(Western blot analysis of CD31 antibody (C-term) in HL60 cell line lysates (35ug/lane). CD31 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of CD31 antibody (C-term) in HL60 cell line lysates (35ug/lane). CD31 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human lung carcinoma with CD31 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human lung carcinoma with CD31 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(CD31 Antibody (C-term) flow cytometric analysis of HL60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(CD31 Antibody (C-term) flow cytometric analysis of HL60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]
Uniprot Description
PECAM-1: a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions. Plays a role in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, cell migration, negative regulation of immune cell signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. Undergoes alternative splicing, generating multiple isoforms in vascular beds of various tissues.
Protein type: Cell adhesion; Membrane protein, integral
Chromosomal Location of Human Ortholog: 17q23.3
Cellular Component: extracellular space; platelet alpha granule membrane; integral to membrane; plasma membrane; cell junction
Molecular Function: protein binding
Biological Process: platelet activation; extracellular matrix organization and biogenesis; platelet degranulation; diapedesis; cell recognition; blood coagulation; signal transduction; cell adhesion; leukocyte migration; phagocytosis
Research Articles on CD31
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Product Notes
The CD31 pecam1 (Catalog #AAA6283073) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD31, CT (PECAM1, Platelet endothelial cell adhesion molecule, EndoCAM, GPIIA', PECA1, CD31) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD31 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 ELISA: 1:1000 IHC: 1:10-50 WB: 1:50-100 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CD31 pecam1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD31, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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