Sheep anti-Mouse, Rat SH2 Domain Protein 2A Polyclonal Antibody | anti-SH2D2A antibody

SH2 Domain Protein 2A (SH2D2A, Lck-associated Adapter Protein, Lad, Rlk/Itk-binding Protein, RIBP)

Cat. Num. AAA628204

• Gene Names: SH2D2A; SCAP; TSAD; VRAP; F2771
• Reactivity: Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: SH2 Domain Protein 2A; Polyclonal Antibody; SH2 Domain Protein 2A (SH2D2A; Lck-associated Adapter Protein; Lad; Rlk/Itk-binding Protein; RIBP); Anti -SH2 Domain Protein 2A (SH2D2A; anti-SH2D2A antibody

• Host: Sheep
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse SH2D2A. Species Crossreactivity: rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with PBS, 0.02% sodium azide.

• Applicable Applications for anti-SH2D2A antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1ug/ml Detects a band at ~45kD.
• Immunogen: Purified, E. coli-derived recombinant mouse RIBP (rmRIBP; aa299-374; Accession # Q9QXK9).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS, 0.02% sodium azide. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

SDS-Page

(Detection of RIBP/SH2D2A: Lysates from mouse CTLL-2 and rat Nb2-11 cells were resolved by SDS-PAGE. Following electrophoresis, proteins were transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml anti-RIBP.)

Related Product Information for anti-SH2D2A antibody

RIBP (Rlk/Itk-binding protein; also SH2D2A, LAD/Lck-associated adapter protein, MEKK2-binding protein and TSAd) is a 45-47kD cytoplasmic adaptor protein. It is found in B cells, thymocytes, NK cells and T cells, and appears to be necessary for TCR-initiated proliferation, plus IL-2 and IFN-gamma secretion. This is likely to be a function of its association with MEKK2 near the T cell receptor interaction site. Mouse RIBP is 374aa in length and contains one SH2 domain (aa116-207), plus a Pro-rich region (aa267-308) that contains an SH3-binding motif (aa267-273). Phosphorylation may occur on Ser237, Ser316 and Tyr325. There is one alternative splice form termed LAD that shows a deletion of aa254-261. Over aa299-374, mouse RIBP shares 79% and 63% aa identity with rat and the presumed human RIBP ortholog (termed TSAd/SH2D2A), respectively.

Product Categories/Family for anti-SH2D2A antibody

Antibodies; Abs to Protein Kinase Substrates

NCBI and Uniprot Product Information

• NCBI GI #: 239787758
• NCBI GeneID: 9047
• NCBI Accession #: NP_001154914.1
• NCBI GenBank Nucleotide #: NM_001161442.1
• UniProt Accession #: Q5UBZ1; Q5UBZ2; Q5UBZ3; Q5VZS4; Q9NP31
• Molecular Weight: 40,951 Da
• NCBI Official Full Name: SH2 domain-containing protein 2A isoform 3
• NCBI Official Synonym Full Names: SH2 domain containing 2A
• NCBI Official Symbol: SH2D2A
• NCBI Official Synonym Symbols: SCAP; TSAD; VRAP; F2771
• NCBI Protein Information: SH2 domain-containing protein 2A; OTTHUMP00000038737; OTTHUMP00000038738; SH2 domain protein 2A; VEGF receptor-associated protein; T cell specific adapter protein TSAd; T lymphocyte specific adaptor protein
• UniProt Protein Name: SH2 domain containing adpater protein 2 transcript variant 2
• Protein Family: SH2 domain-containing protein
• UniProt Gene Name: SH2D2A
• UniProt Entry Name: Q5UBZ1_HUMAN

NCBI Description

This gene encodes an adaptor protein thought to function in T-cell signal transduction. A related protein in mouse is responsible for the activation of lymphocyte-specific protein-tyrosine kinase and functions in downstream signaling. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Uniprot Description

TSAd: an adaptor protein that may facilitate and regulate interaction of KDR with effector proteins important to endothelial cell survival and proliferation. May play a role in the CD4-p56- LCK-dependent signal transduction pathway in T cells. Could also play an important role in normal and pathological angiogenesis.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: cytoplasm; cytosol

Molecular Function: protein binding; SH3/SH2 adaptor activity; SH3 domain binding

Biological Process: cell proliferation; positive regulation of signal transduction; angiogenesis; vascular endothelial growth factor receptor signaling pathway; signal transduction; cell differentiation