Rabbit anti-Human CBR3 Polyclonal Antibody | anti-CBR3 antibody

CBR3, CT (CBR3, Carbonyl reductase [NADPH] 3, NADPH-dependent carbonyl reductase 3) (APC)

Cat. Num. AAA6281146

• Gene Names: CBR3; hCBR3; SDR21C2
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: CBR3; Polyclonal Antibody; CT (CBR3; Carbonyl reductase [NADPH] 3; NADPH-dependent carbonyl reductase 3) (APC); NADPH-dependent carbonyl reductase 3; anti-CBR3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-CBR3 antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: CBR3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 194-224 amino acids from the C-terminal region of human CBR3.
• Conjugate: APC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CBR3 (arrow) using rabbit polyclonal CBR3 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CBR3 gene.)

Flow Cytometry (FC/FACS)

(CBR3 Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CBR3 antibody

Carbonyl reductase 3 catalyzes the reduction of a large number of biologically and pharmacologically active carbonyl compounds to their corresponding alcohols. The enzyme is classified as a monomeric NADPH-dependent oxidoreductase. CBR3 contains three exons spanning 11.2 kilobases and is closely linked to another carbonyl reductase gene-CBR1.

Product Categories/Family for anti-CBR3 antibody

Antibodies; Enzymes; Reductase

References

Guey, L.T., et al. Eur. Urol. 57(2):283-292(2010). Hosgood, H.D. III, et al. Respir Med 103(12):1866-1870(2009). Zhang, J., et al. Pharm. Res. 26(9):2209-2215(2009). Choi, J.Y., et al. Clin. Cancer Res. 15(16):5258-5266(2009). Pilka, E.S., et al. PLoS ONE 4 (10), E7113 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4502601
• NCBI GeneID: 874
• NCBI Accession #: NP_001227.1
• NCBI GenBank Nucleotide #: NM_001236.3
• UniProt Accession #: O75828; Q6FHP2
• Molecular Weight: 30,850 Da
• NCBI Official Full Name: carbonyl reductase
• NCBI Official Synonym Full Names: carbonyl reductase 3
• NCBI Official Symbol: CBR3
• NCBI Official Synonym Symbols: hCBR3; SDR21C2
• NCBI Protein Information: carbonyl reductase [NADPH] 3; carbonyl reductase [NADPH] 3; carbonyl reductase (NADPH) 3; NADPH-dependent carbonyl reductase 3; short chain dehydrogenase/reductase family 21C, member 2
• UniProt Protein Name: Carbonyl reductase [NADPH] 3
• Protein Family: Carbonyl reductase
• UniProt Gene Name: CBR3
• UniProt Entry Name: CBR3_HUMAN

NCBI Description

Carbonyl reductase 3 catalyzes the reduction of a large number of biologically and pharmacologically active carbonyl compounds to their corresponding alcohols. The enzyme is classified as a monomeric NADPH-dependent oxidoreductase. CBR3 contains three exons spanning 11.2 kilobases and is closely linked to another carbonyl reductase gene - CBR1. [provided by RefSeq, Jul 2008]

Uniprot Description

CBR3: Has low NADPH-dependent oxidoreductase activity towards 4-benzoylpyridine and menadione (in vitro). Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Protein type: Oxidoreductase; EC 1.1.1.184; Lipid Metabolism - arachidonic acid

Chromosomal Location of Human Ortholog: 21q22.2

Cellular Component: nucleoplasm; extracellular space; cytoplasm; cytosol

Molecular Function: 3-keto sterol reductase activity; carbonyl reductase (NADPH) activity

Biological Process: phylloquinone catabolic process; cognition