Rabbit anti-Human CBAA1 Polyclonal Antibody | anti-CBAA1 antibody

CBAA1, NT (MICU1, CALC, CBARA1, Calcium uptake protein 1, mitochondrial, Atopy-related autoantigen CALC, Calcium-binding atopy-related autoantigen 1, Allergen=Hom s 4) (Azide free) (HRP)

Cat. Num. AAA6281094

• Gene Names: MICU1; CALC; EFHA3; MPXPS; CBARA1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CBAA1; Polyclonal Antibody; NT (MICU1; CALC; CBARA1; Calcium uptake protein 1; mitochondrial; Atopy-related autoantigen CALC; Calcium-binding atopy-related autoantigen 1; Allergen=Hom s 4) (Azide free) (HRP); MICU1; Allergen=Hom s 4; anti-CBAA1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-CBAA1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CBAA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59~89 amino acids from the N-terminal region of human CBAA1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CBAA1 Antibody (N-term) in K562 cell line lysates (35ug/lane). CBAA1 (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-CBAA1 antibody

Antibodies; Mitochondrial Proteins

References

Bordicchia, M., et al. Metab. Clin. Exp. (2009). In press: Grupe, A., et al. Am. J. Hum. Genet. 78(1):78-88(2006). Natter, S., et al. FASEB J. 12(14):1559-1569(1998).

NCBI and Uniprot Product Information

• NCBI GI #: 116256483
• NCBI GeneID: 10367
• NCBI Accession #: NP_006068.2
• NCBI GenBank Nucleotide #: NM_006077.3
• UniProt Accession #: Q9BPX6; O75785; Q9H9N6; Q9UFX0; A8MV96; B3KN20; B4DJH9; B4DPI1; B5MBY3; D3YTJ3
• Molecular Weight: 31,931 Da
• NCBI Official Full Name: calcium uptake protein 1, mitochondrial isoform 1
• NCBI Official Synonym Full Names: mitochondrial calcium uptake 1
• NCBI Official Symbol: MICU1
• NCBI Official Synonym Symbols: CALC; EFHA3; MPXPS; CBARA1
• NCBI Protein Information: calcium uptake protein 1, mitochondrial; ara CALC; atopy-related autoantigen CALC; calcium-binding atopy-related autoantigen 1
• UniProt Protein Name: Calcium uptake protein 1, mitochondrial
• UniProt Gene Name: MICU1
• UniProt Synonym Gene Names: CALC; CBARA1; ara CALC
• UniProt Entry Name: MICU1_HUMAN

NCBI Description

This gene encodes an essential regulator of mitochondrial Ca2+ uptake under basal conditions. The encoded protein interacts with the mitochondrial calcium uniporter, a mitochondrial inner membrane Ca2+ channel, and is essential in preventing mitochondrial Ca2+ overload, which can cause excessive production of reactive oxygen species and cell stress. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Mar 2013]

Uniprot Description

MICU1: Key regulator of mitochondrial calcium uptake required for calcium entry into mitochondrion. May act as a calcium sensor via its EF-hand domains, gating the activity of a calcium channel partner. Induces T-helper 1-mediated autoreactivity, which is accompanied by the release of IFNG. Belongs to the MICU1 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Mitochondrial; Calcium-binding

Chromosomal Location of Human Ortholog: 10q22.1

Cellular Component: mitochondrion; mitochondrial inner membrane; mitochondrial intermembrane space; intracellular

Molecular Function: identical protein binding; protein binding; protein heterodimerization activity; calcium ion binding

Biological Process: mitochondrial calcium ion homeostasis; defense response; mitochondrial calcium ion transport; protein homooligomerization; elevation of mitochondrial calcium ion concentration

Disease: Myopathy With Extrapyramidal Signs