Rabbit anti-Human Calcitonin Polyclonal Antibody | anti-CALCA antibody

Calcitonin, CT (CALCA, CALC1, Calcitonin, Katacalcin, Calcitonin carboxyl-terminal peptide, PDN-21) (MaxLight 650)

Cat. Num. AAA6280504

• Gene Names: CALCA; CT; KC; PCT; CGRP; CALC1; CGRP1; CGRP-I
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: Calcitonin; Polyclonal Antibody; CT (CALCA; CALC1; Katacalcin; Calcitonin carboxyl-terminal peptide; PDN-21) (MaxLight 650); CALCA; PDN-21; anti-CALCA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Calcitonin.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-CALCA antibody: Western Blot (WB), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 104-132 in the C-terminal region of human Calcitonin, UniProt Accession #P01258.
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in MDA-MB231 cell line lysates (35ug/lane) usingMBS6013452. This demonstrates that MBS6013452 detected the CALCA/CT protein (arrow).)

Related Product Information for anti-CALCA antibody

This gene encodes the peptide hormones calcitonin, calcitonin gene-related peptide and katacalcin by tissue-specific alternative RNA splicing of the gene transcripts and cleavage of inactive precursor proteins. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-CALCA antibody

Antibodies; Calcitonin

References

1. Giroux, S., et al. Bone 47(5):975-981 (2010). 2. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253 (2010). 3. Lee, J.Y., et al. Korean J Lab Med 30(4):406-413 (2010). 4. Giovanella, L., et al. Clin. Chem. Lab. Med. 48(8):1163-1165 (2010). 5. Hatzistilianou, M. ScientificWorldJournal 10, 1941-1946 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 4502545
• NCBI GeneID: 796
• NCBI Accession #: NP_001732.1; NP_001029124.1
• NCBI GenBank Nucleotide #: NM_001741.2
• UniProt Accession #: P01258; Q13935; Q13937; Q52LX7; B7ZL39; P06881
• Molecular Weight: 13,898 Da
• NCBI Official Full Name: calcitonin isoform CT preproprotein
• NCBI Official Synonym Full Names: calcitonin-related polypeptide alpha
• NCBI Official Symbol: CALCA
• NCBI Official Synonym Symbols: CT; KC; PCT; CGRP; CALC1; CGRP1; CGRP-I
• NCBI Protein Information: calcitonin; calcitonin gene-related peptide 1
• UniProt Protein Name: Calcitonin
• Protein Family: Calcitonin
• UniProt Gene Name: CALCA
• UniProt Synonym Gene Names: CALC1; CCP
• UniProt Entry Name: CALC_HUMAN

NCBI Description

This gene encodes the peptide hormones calcitonin, calcitonin gene-related peptide and katacalcin by tissue-specific alternative RNA splicing of the gene transcripts and cleavage of inactive precursor proteins. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator and as an antimicrobial peptide while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2014]

Uniprot Description

CALCA: Calcitonin causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones. Belongs to the calcitonin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 11p15.2

Cellular Component: extracellular space; cell soma; cytoplasm; extracellular region; terminal button; nucleus

Molecular Function: identical protein binding; protein binding; calcitonin receptor binding; hormone activity; protein complex binding; receptor binding

Biological Process: negative regulation of osteoclast differentiation; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of interleukin-1 alpha production; vasodilation of artery during baroreceptor response to increased systemic arterial blood pressure; response to pain; positive regulation of vasodilation; protein amino acid phosphorylation; G-protein signaling, adenylate cyclase activating pathway; positive regulation of interleukin-8 production; monocyte chemotaxis; elevation of cytosolic calcium ion concentration; leukocyte adhesion; cell-cell signaling; vasculature development; neuropeptide signaling pathway; negative regulation of smooth muscle contraction; receptor internalization; regulation of blood pressure; positive regulation of cAMP biosynthetic process; negative regulation of ossification; feeding behavior; negative regulation of blood pressure; positive regulation of adenylate cyclase activity; positive regulation of macrophage differentiation; negative regulation of bone resorption; detection of temperature stimulus involved in sensory perception of pain; inflammatory response; negative regulation of neurological process; aging; cytosolic calcium ion homeostasis; regulation of heart rate; adenylate cyclase activation; positive regulation of ossification; regulation of systemic arterial blood pressure by neurological process; G-protein coupled receptor internalization; response to heat; endothelial cell proliferation; regulation of inflammatory response; activation of protein kinase activity; endothelial cell migration; negative regulation of transcription, DNA-dependent; embryo implantation