Rabbit anti-Human CAD Polyclonal Antibody | anti-CAD antibody

CAD, ID (CAD, CAD protein, Glutamine-dependent carbamoyl-phosphate synthase, Aspartate carbamoyltransferase, Dihydroorotase) (Azide free) (HRP)

Cat. Num. AAA6280434

• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CAD; Polyclonal Antibody; ID (CAD; CAD protein; Glutamine-dependent carbamoyl-phosphate synthase; Aspartate carbamoyltransferase; Dihydroorotase) (Azide free) (HRP); Dihydroorotase; anti-CAD antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-CAD antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CAD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 787-816 amino acids from the Central region of human CAD.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CAD Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the CAD antibody detected the CAD protein (arrow).)

Immunohistochemistry (IHC)

(CAD Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CAD Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(CAD Antibody (Center) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-CAD antibody

Antibodies; Enzymes; Synthase

References

Jia, P., et al. Schizophr. Res. 122 (1-3), 38-42 (2010): Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Ahuja, V., et al. J. Inherit. Metab. Dis. 31(4):481-491(2008). Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007). Olsen, J.V., et al. Cell 127(3):635-648(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 18105007
• NCBI GeneID: 790
• NCBI Accession #: NP_004332.2
• NCBI GenBank Nucleotide #: NM_004341.3
• UniProt Accession #: P27708; Q6P0Q0; D6W552
• Molecular Weight: 242,984 Da
• NCBI Official Full Name: CAD protein
• NCBI Official Synonym Full Names: carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
• NCBI Official Symbol: CAD
• NCBI Protein Information: CAD protein; CAD trifunctional protein; multifunctional protein CAD
• UniProt Protein Name: CAD protein
• Protein Family: Cadherin
• UniProt Gene Name: CAD
• UniProt Entry Name: PYR1_HUMAN

NCBI Description

The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. [provided by RefSeq, Jul 2008]

Uniprot Description

CAD: a multifunctional enzyme that catalyzes four enzymatic activities of the pyrimidine pathway, including the rate-limiting first step. The four enzymatic activities involved in de novo pyrimidine synthesis are glutamine amidotransferase, carbamoyl-phosphate synthetase, aspartate transcarbamoylase, and dihydroorotase activities. Provides essential precursors for DNA synthesis. Required for the de novo synthesis of pyrimidine nucleotides and cell growth. Phosphorylation by p70S6K promotes CAD oligomerization, stimulating de novo synthesis of pyrimidines and progression through S phase in mammalian cells. Utilizes glutamine, bicarbonate, and aspartic acid to form a pyrimidine ring that is subsequently attached to ribose to generate a pyrimidine.

Protein type: EC 6.3.5.5; EC 2.1.3.2; Transferase; Ligase; Amino Acid Metabolism - alanine, aspartate and glutamate; EC 3.5.2.3; Hydrolase; Nucleotide Metabolism - pyrimidine

Chromosomal Location of Human Ortholog: 2p22-p21

Cellular Component: nucleoplasm; cell projection; protein complex; nuclear matrix; membrane; cell soma; plasma membrane; terminal button; nucleus; cytosol

Molecular Function: carbamoyl-phosphate synthase (ammonia) activity; identical protein binding; enzyme binding; zinc ion binding; carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity; aspartate carbamoyltransferase activity; ATP binding; protein kinase activity; dihydroorotase activity

Biological Process: lactation; organ regeneration; arginine biosynthetic process; nucleobase, nucleoside and nucleotide metabolic process; protein amino acid autophosphorylation; heart development; UTP biosynthetic process; peptidyl-threonine phosphorylation; glutamine metabolic process; response to testosterone stimulus; female pregnancy; liver development; response to caffeine; 'de novo' pyrimidine base biosynthetic process; response to cortisol stimulus; pyrimidine base metabolic process; pyrimidine nucleoside biosynthetic process; glutamine catabolic process; drug metabolic process; urea cycle; response to amine stimulus

Disease: Congenital Disorder Of Glycosylation, Type Iz