Western Blot (WB)
(BCAS2 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the BCAS2 antibody detected the BCAS2 protein (arrow).)
Rabbit anti-Human BCAS2 Polyclonal Antibody | anti-BCAS2 antibody
BCAS2, NT (BCAS2, DAM1, Pre-mRNA-splicing factor SPF27, Breast carcinoma-amplified sequence 2, DNA amplified in mammary carcinoma 1 protein, Spliceosome-associated protein SPF 27) (PE)
Gene Names
BCAS2; DAM1; SPF27; Snt309
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
BCAS2; Polyclonal Antibody; NT (BCAS2; DAM1; Pre-mRNA-splicing factor SPF27; Breast carcinoma-amplified sequence 2; DNA amplified in mammary carcinoma 1 protein; Spliceosome-associated protein SPF 27) (PE); Spliceosome-associated protein SPF 27; anti-BCAS2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-BCAS2 antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
BCAS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 20-49 amino acids from the N-terminal region of human BCAS2.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(BCAS2 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the BCAS2 antibody detected the BCAS2 protein (arrow).)
Western Blot (WB)
(BCAS2 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the BCAS2 antibody detected the BCAS2 protein (arrow).)
Related Product Information for anti-BCAS2 antibody
BCAS2 is involved in mRNA splicing (By similarity).
Product Categories/Family for anti-BCAS2 antibody
References
Kuo, P.C., et al. Cancer Res. 69(23):8877-8885(2009). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007): Qi, C., et al. Biochem. Biophys. Res. Commun. 328(2):393-398(2005). Lee, S., et al. Cancer Lett. 188 (1-2), 121-126 (2002): Maass, N., et al. Cancer Lett. 185(2):219-223(2002).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26,131 Da
NCBI Official Full Name
pre-mRNA-splicing factor SPF27
NCBI Official Synonym Full Names
breast carcinoma amplified sequence 2
NCBI Official Symbol
BCAS2
NCBI Official Synonym Symbols
DAM1; SPF27; Snt309
NCBI Protein Information
pre-mRNA-splicing factor SPF27; breast carcinoma-amplified sequence 2; spliceosome-associated protein SPF 27; DNA amplified in mammary carcinoma 1 protein; spliceosome associated protein, amplified in breast cancer
UniProt Protein Name
Pre-mRNA-splicing factor SPF27
UniProt Gene Name
BCAS2
UniProt Synonym Gene Names
DAM1
UniProt Entry Name
SPF27_HUMAN
Uniprot Description
BCAS2: Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex. Belongs to the SPF27 family.
Protein type: RNA splicing; Spliceosome; Nuclear receptor co-regulator; Nucleolus
Chromosomal Location of Human Ortholog: 1p13.2
Cellular Component: nucleoplasm; spliceosome; DNA replication factor A complex; nucleolus; nucleus; cell junction
Molecular Function: protein binding
Biological Process: RNA splicing, via transesterification reactions; nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression
Research Articles on BCAS2
Similar Products
Product Notes
The BCAS2 bcas2 (Catalog #AAA6277800) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BCAS2, NT (BCAS2, DAM1, Pre-mRNA-splicing factor SPF27, Breast carcinoma-amplified sequence 2, DNA amplified in mammary carcinoma 1 protein, Spliceosome-associated protein SPF 27) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BCAS2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the BCAS2 bcas2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BCAS2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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