Western Blot (WB)
(GPLD2-R694 western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the GPLD2 antibody detected the GPLD2 protein (arrow).)
Rabbit anti-Human GPLD1 Polyclonal Antibody | anti-GPLD1 antibody
GPLD1, CT (Phosphatidylinositol-glycan-specific Phospholipase D, PI-G PLD, Glycoprotein Phospholipase D, Glycosyl-phosphatidylinositol-specific Phospholipase D, GPI-specific Phospholipase D, GPI-PLD, PIGPLD1)
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500
Western Blot (WB)
(GPLD2-R694 western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the GPLD2 antibody detected the GPLD2 protein (arrow).)
Western Blot (WB)
(GPLD2-R694 western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the GPLD2 antibody detected the GPLD2 protein (arrow).)
Immunohistochemistry (IHC)
(GPLD1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GPLD1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(GPLD1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GPLD1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
NCBI and Uniprot Product Information
NCBI Description
Many proteins are tethered to the extracellular face of eukaryotic plasma membranes by a glycosylphosphatidylinositol (GPI) anchor. The GPI-anchor is a glycolipid found on many blood cells. The protein encoded by this gene is a GPI degrading enzyme. Glycosylphosphatidylinositol specific phospholipase D1 hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans, thereby releasing the attached protein from the plasma membrane. [provided by RefSeq, Jul 2008]
Uniprot Description
GPLD1: This protein hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans (GPI-anchor) thus releasing these proteins from the membrane. Belongs to the GPLD1 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Glycan Metabolism - glycosylphosphatidylinositol (GPI)-anchor biosynthesis; Secreted, signal peptide; Secreted; Phospholipase; EC 3.1.4.50
Chromosomal Location of Human Ortholog: 6p22.1
Cellular Component: proteinaceous extracellular matrix; extracellular space; intracellular membrane-bound organelle; lysosomal membrane; cytoplasm; intracellular
Molecular Function: sodium channel regulator activity; glycosylphosphatidylinositol phospholipase D activity; phospholipase D activity
Biological Process: ossification; GPI anchor release; positive regulation of apoptosis; complement receptor mediated signaling pathway; positive regulation of membrane protein ectodomain proteolysis; phosphatidylcholine metabolic process; negative regulation of cell proliferation; cell migration during sprouting angiogenesis; cellular response to insulin stimulus; insulin receptor signaling pathway; response to glucose stimulus; positive regulation of secretion; chondrocyte differentiation; positive regulation of cytolysis
Research Articles on GPLD1
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Product Notes
The GPLD1 gpld1 (Catalog #AAA627733) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GPLD1, CT (Phosphatidylinositol-glycan-specific Phospholipase D, PI-G PLD, Glycoprotein Phospholipase D, Glycosyl-phosphatidylinositol-specific Phospholipase D, GPI-specific Phospholipase D, GPI-PLD, PIGPLD1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GPLD1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 1:1,000 Western Blot: 1:100-1:500. Researchers should empirically determine the suitability of the GPLD1 gpld1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPLD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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