Rabbit anti-Human ASCL1 Polyclonal Antibody | anti-ASCL1 antibody

ASCL1, NT (ASCL1, ASH1, BHLHA46, HASH1, Achaete-scute homolog 1, Class A basic helix-loop-helix protein 46) (AP)

Cat. Num. AAA6275589

• Gene Names: ASCL1; ASH1; HASH1; MASH1; bHLHa46
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ASCL1; Polyclonal Antibody; NT (ASCL1; ASH1; BHLHA46; HASH1; Achaete-scute homolog 1; Class A basic helix-loop-helix protein 46) (AP); Class A basic helix-loop-helix protein 46; anti-ASCL1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-ASCL1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ASCL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63-90 amino acids from the N-terminal region of human ASCL1.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(ASCL1 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the ASCL1 antibody detected the ASCL1 protein (arrow).)

Related Product Information for anti-ASCL1 antibody

This gene encodes a member of the basic helix-loop-helix (BHLH) family of transcription factors. The protein activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. This protein plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. Mutations in this gene may contribute to the congenital central hypoventilation syndrome (CCHS) phenotype in rare cases.

Product Categories/Family for anti-ASCL1 antibody

Antibodies; Transcription Factors

References

Phi, J.H., et al. J Neurosurg Pediatr 5(6):608-614(2010). McGaughey, D.M., et al. Genomics 95(6):363-369(2010). Li, F., et al. Zhongguo Fei Ai Za Zhi 13(4):317-321(2010). Deng, H., et al. Biochem. Biophys. Res. Commun. 392(4):548-550(2010). Improgo, M.R., et al. Mol. Cancer Res. 8(2):194-203(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 55743094
• NCBI GeneID: 429
• NCBI Accession #: NP_004307.2
• NCBI GenBank Nucleotide #: NM_004316.3
• UniProt Accession #: P50553; Q9BQ30; A8K3C4
• Molecular Weight: 25,454 Da
• NCBI Official Full Name: achaete-scute homolog 1
• NCBI Official Synonym Full Names: achaete-scute family bHLH transcription factor 1
• NCBI Official Symbol: ASCL1
• NCBI Official Synonym Symbols: ASH1; HASH1; MASH1; bHLHa46
• NCBI Protein Information: achaete-scute homolog 1
• UniProt Protein Name: Achaete-scute homolog 1
• Protein Family: Achaete-scute
• UniProt Gene Name: ASCL1
• UniProt Synonym Gene Names: ASH1; BHLHA46ImportedManual assertion inferred from database entriesiHGNC:738; HASH11 PublicationManual assertion based on opinion iniRef.1; bHLHa46ImportedManual assertion inferred from database entriesiHGNC:738

NCBI Description

This gene encodes a member of the basic helix-loop-helix (BHLH) family of transcription factors. The protein activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. This protein plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. Mutations in this gene may contribute to the congenital central hypoventilation syndrome (CCHS) phenotype in rare cases. [provided by RefSeq, Jul 2008]

Uniprot Description

Transcription factor that plays a key role in neuronal differentiation: acts as a pioneer transcription factor, accessing closed chromatin to allow other factors to bind and activate neural pathways. Directly binds the E box motif (5'-CANNTG-3') on promoters and promotes transcription of neuronal genes. The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro. Plays a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Acts synergistically with FOXN4 to specify the identity of V2b neurons rather than V2a from bipotential p2 progenitors during spinal cord neurogenesis, probably through DLL4-NOTCH signaling activation.