Western Blot (WB)
(ARHGEF35 Antibody (Center) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the ARHGEF35 antibody detected the ARHGEF35 protein (arrow).)
Rabbit anti-Human ARHGEF35 Polyclonal Antibody | anti-ARHGEF35 antibody
ARHGEF35, ID (ARHGEF35, ARHGEF5L, Rho guanine nucleotide exchange factor 35, Rho guanine nucleotide exchange factor 5-like protein) (AP)
Gene Names
ARHGEF35; ARHGEF5L
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
ARHGEF35; Polyclonal Antibody; ID (ARHGEF35; ARHGEF5L; Rho guanine nucleotide exchange factor 35; Rho guanine nucleotide exchange factor 5-like protein) (AP); Rho guanine nucleotide exchange factor 5-like protein; anti-ARHGEF35 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-ARHGEF35 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
ARHGEF35 antibody is generated from rabbits immunized with a KLH conjugated synthetic between 247-275 amino acids selected from the Central region of human ARHGEF35.
Conjugate
AP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(ARHGEF35 Antibody (Center) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the ARHGEF35 antibody detected the ARHGEF35 protein (arrow).)
Western Blot (WB)
(ARHGEF35 Antibody (Center) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the ARHGEF35 antibody detected the ARHGEF35 protein (arrow).)
Product Categories/Family for anti-ARHGEF35 antibody
References
Kimura, K., et al. Genome Res. 16(1):55-65(2006). Strausberg, R.L., et al. Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903(2002).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
53,287 Da
NCBI Official Full Name
rho guanine nucleotide exchange factor 35
NCBI Official Synonym Full Names
Rho guanine nucleotide exchange factor 35
NCBI Official Symbol
ARHGEF35
NCBI Official Synonym Symbols
ARHGEF5L
NCBI Protein Information
rho guanine nucleotide exchange factor 35
UniProt Protein Name
Rho guanine nucleotide exchange factor 35
Protein Family
UniProt Gene Name
ARHGEF35
UniProt Synonym Gene Names
ARHGEF5L
Uniprot Description
Enzyme and pathway databasesReactome - a knowledgebase of biological pathways and processesMore...ReactomeiR-HSA-193648. NRAGE signals death through JNK. R-HSA-194840. Rho GTPase cycle. R-HSA-416482. G alpha (12/13) signalling events.
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Product Notes
The ARHGEF35 arhgef35 (Catalog #AAA6275006) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ARHGEF35, ID (ARHGEF35, ARHGEF5L, Rho guanine nucleotide exchange factor 35, Rho guanine nucleotide exchange factor 5-like protein) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ARHGEF35 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ARHGEF35 arhgef35 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARHGEF35, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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