Rabbit anti-Human APG4B Polyclonal Antibody | anti-APG4B antibody

APG4B, ID (G254) (ATG4B, APG4B, AUTL1, KIAA0943, Cysteine protease ATG4B, AUT-like 1 cysteine endopeptidase, Autophagin-1, Autophagy-related cysteine endopeptidase 1, Autophagy-related protein 4 homolog B) (Biotin)

Cat. Num. AAA6274238

• Gene Names: ATG4B; APG4B; AUTL1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: APG4B; Polyclonal Antibody; ID (G254) (ATG4B; AUTL1; KIAA0943; Cysteine protease ATG4B; AUT-like 1 cysteine endopeptidase; Autophagin-1; Autophagy-related cysteine endopeptidase 1; Autophagy-related protein 4 homolog B) (Biotin); ATG4B; Autophagy-related protein 4 homolog B; anti-APG4B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-APG4B antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: APG4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 239-265 amino acids from the Central region of human Autophagy APG4B.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(APG4B Antibody (Center G254) western blot analysis in Uterus tissue lysates (35ug/lane).This demonstrates the APG4B antibody detected the APG4B protein (arrow).)

Related Product Information for anti-APG4B antibody

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq].

Product Categories/Family for anti-APG4B antibody

Antibodies; Enzymes; Protease (Proteinase; Peptidase)

References

Satoo, K., et al. EMBO J. 28(9):1341-1350(2009). Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007): Olsen, J.V., et al. Cell 127(3):635-648(2006). Olsen, J.V., et al. Cell 127(3):635-648(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 47132611
• NCBI GeneID: 23192
• NCBI Accession #: NP_037457
• NCBI GenBank Nucleotide #: NM_013325.4
• UniProt Accession #: Q9Y4P1; Q53NU4; Q6ZUV8; Q8WYM9; Q96K07; Q96K96; Q96SZ1; Q9Y2F2; B7WNK2
• Molecular Weight: Predicted: 43 kDa; ; Observed: 44 kDa
• NCBI Official Full Name: cysteine protease ATG4B isoform a
• NCBI Official Synonym Full Names: autophagy related 4B, cysteine peptidase
• NCBI Official Symbol: ATG4B
• NCBI Official Synonym Symbols: APG4B; AUTL1
• NCBI Protein Information: cysteine protease ATG4B; hAPG4B; autophagin-1; APG4 autophagy 4 homolog B; AUT-like 1 cysteine endopeptidase; ATG4 autophagy related 4 homolog B; autophagy-related protein 4 homolog B; autophagy-related cysteine endopeptidase 1
• UniProt Protein Name: Cysteine protease ATG4B
• UniProt Gene Name: ATG4B
• UniProt Synonym Gene Names: APG4B; AUTL1; KIAA0943; hAPG4B
• UniProt Entry Name: ATG4B_HUMAN

NCBI Description

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

ATG4B: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas. Inhibited by N-ethylmaleimide. Belongs to the peptidase C54 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy; Protease; EC 3.4.22.-; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: cytoplasm; cytosol

Molecular Function: protein binding; cysteine-type endopeptidase activity; endopeptidase activity; cysteine-type peptidase activity

Biological Process: protein delipidation; mitochondrion degradation; positive regulation of protein catabolic process; autophagy; C-terminal protein lipidation; protein processing; protein targeting to membrane; proteolysis; positive regulation of autophagy; autophagic vacuole formation; cellular response to nitrogen starvation