Rabbit anti-Human ANO7 Polyclonal Antibody | anti-ANO7 antibody

ANO7, NT (ANO7, NGEP, PCANAP5, TMEM16G, Anoctamin-7, Dresden transmembrane protein of the prostate, IPCA-5, New gene expressed in prostate, Prostate cancer-associated protein 5, Transmembrane protein 16G) (FITC)

Cat. Num. AAA6273821

• Gene Names: ANO7; NGEP; DTMPP; IPCA5; D-TMPP; IPCA-5; PCANAP5; TMEM16G; PCANAP5L
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ANO7; Polyclonal Antibody; NT (ANO7; NGEP; PCANAP5; TMEM16G; Anoctamin-7; Dresden transmembrane protein of the prostate; IPCA-5; New gene expressed in prostate; Prostate cancer-associated protein 5; Transmembrane protein 16G) (FITC); Transmembrane protein 16G; anti-ANO7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ANO7.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-ANO7 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 106-135 in the N-terminal region of human ANO7, UniProt Accession #Q6IWH7.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in MCF-7 cell line lysates (35ug/lane) usingMBS6005848. ANO7 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(Flow Cytometry analysis of MCF-7 cells using MBS6005848 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ANO7 antibody

ANO7 may act as a calcium-activated chloride channel. This protein May play a role in cell-cell interactions.

Product Categories/Family for anti-ANO7 antibody

Antibodies; Transmembrane Proteins

References

1. Cereda, V., et al. Cancer Immunol. Immunother. 59(1):63-71(2010). 2. Hartzell, H.C., et al. J. Physiol. (Lond.) 587 (PT 10), 2127-2139 (2009). 3. Das, S., et al. Cancer Res. 68(15):6306-6312(2008). 4. Kiessling, A., et al. Prostate 64(4):387-400(2005). 5. Katoh, M., et al. Int. J. Mol. Med. 14(4):759-764(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 48717269
• NCBI GeneID: 50636
• NCBI Accession #: NP_001001666.1
• NCBI GenBank Nucleotide #: NM_001001666.3
• UniProt Accession #: Q6IWH7; Q6IWH6
• Molecular Weight: 105,532 Da
• NCBI Official Full Name: anoctamin-7 isoform NGEP-short
• NCBI Official Synonym Full Names: anoctamin 7
• NCBI Official Symbol: ANO7
• NCBI Official Synonym Symbols: NGEP; DTMPP; IPCA5; D-TMPP; IPCA-5; PCANAP5; TMEM16G; PCANAP5L
• NCBI Protein Information: anoctamin-7; transmembrane protein 16G; prostate cancer associated protein 5; Dresden-transmembrane protein of the prostate
• UniProt Protein Name: Anoctamin-7
• Protein Family: Anoctamin
• UniProt Gene Name: ANO7
• UniProt Synonym Gene Names: NGEP; PCANAP5; TMEM16G; D-TMPP
• UniProt Entry Name: ANO7_HUMAN

NCBI Description

This prostate-specific gene encodes a cytoplasmic protein, as well as a polytopic membrane protein which may serve as a target in prostate cancer diagnosis and immunotherapy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2011]

Uniprot Description

ANO7: May act as a calcium-activated chloride channel. May play a role in cell-cell interactions. Belongs to the anoctamin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter, ion channel; Channel, chloride; Calcium-binding; Membrane protein, integral; Transporter; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: endoplasmic reticulum; integral to membrane; plasma membrane; intracellular; nucleus; cytosol; cell junction

Molecular Function: phospholipid scramblase activity; intracellular calcium activated chloride channel activity

Biological Process: chloride transport; lipid transport; transmembrane transport