Sheep anti-Human Proteinase 3 Polyclonal Antibody | anti-PRTN3 antibody

Proteinase 3 (Proteinase-3, PR3, PR-3, PRTN3, ACPA, Azurophil Granule Protein 7, AGP7, cANCA, c-ANCA Antigen, EC 3.4.21.76, Leukocyte Proteinase 3, MBT, Myeloblastin, MBN, Neutrophil Proteinase 4, NP4, NP-4, p29, Wegener Autoantigen)

Cat. Num. AAA627376

• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Proteinase 3; Polyclonal Antibody; Proteinase 3 (Proteinase-3; PR3; PR-3; PRTN3; ACPA; Azurophil Granule Protein 7; AGP7; cANCA; c-ANCA Antigen; EC 3.4.21.76; Leukocyte Proteinase 3; MBT; Myeloblastin; MBN; Neutrophil Proteinase 4; NP4; NP-4; p29; Wegener Autoantigen); Anti -Proteinase 3 (Proteinase-3; anti-PRTN3 antibody

• Host: Sheep
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PRTN3.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with sterile PBS.

• Applicable Applications for anti-PRTN3 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Dilution: ELISA:0.5-1ug/ml; Western Blot:1ug/ml
• Immunogen: Purified, Sf 21-derived, recombinant human Proteinase 3 (aa26-249; Accession #P24158, with Val119Ile and Ser203Ala substitutions).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-PRTN3 antibody

Proteinase 3 (PRTN3; also PR3/leukocyte, Myeloblastin and NP-4) is a 32-33kD member of the peptidase S1 family of enzymes. It is expressed by monocytes and neutrophils, the latter of which either secretes it, sequesters it in azurophilic granules, or expresses it on the cell surface. When secreted, it acts on HK and activates the kinin pathway. In azurophilic granules, it aids in the digestion of phagocytosed material. On the cell surface, it likely acts on ECM. Human PRTN3 proprecursor is 231aa in length. It contains an Ala26Glu27 propeptide that is removed during maturation, a 221aa mature enzyme (aa28-248), and an eight aa C-terminal propeptide (aa249-256). Within the cell, a 35kD immature form exists; on the cell surface, both constitutively inactive, and induced active forms may be found, often in a noncovalent association with CD177/NB1. Over aa26-249, human PRTN3 shares 68% aa identity with mouse PRTN3.

Product Categories/Family for anti-PRTN3 antibody

Antibodies; Abs to Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 1335280
• UniProt Accession #: P24158; P15637; P18078; Q4VB08; Q4VB09; Q6LBM7; Q6LBN2; Q9UD25; Q9UQD8
• Molecular Weight: 27,807 Da
• NCBI Official Full Name: proteinase 3
• UniProt Protein Name: Myeloblastin
• Protein Family: Myeloblastin
• UniProt Gene Name: PRTN3
• UniProt Synonym Gene Names: MBN; PR-3; PR3; NP-4
• UniProt Entry Name: PRTN3_HUMAN

Uniprot Description

PRTN3: Polymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to hamsters. Belongs to the peptidase S1 family. Elastase subfamily.

Protein type: Cell cycle regulation; Protease; EC 3.4.21.76

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: extracellular matrix; extracellular space; plasma membrane; cytosol

Molecular Function: enzyme binding; serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: negative regulation of phagocytosis; collagen catabolic process; positive regulation of cell proliferation; blood coagulation; proteolysis