Rabbit anti-Human HRH3 Polyclonal Antibody | anti-Hrh3 antibody

HRH3, CT (Histamine H3 Receptor, HH3R, G-protein Coupled Receptor 97, HRH3, GPCR97)

Cat. Num. AAA627344

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: HRH3; Polyclonal Antibody; CT (Histamine H3 Receptor; HH3R; G-protein Coupled Receptor 97; GPCR97); Anti -HRH3; anti-Hrh3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HRH3 at ~50kD.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-Hrh3 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1:100-1:500; Immunohistochemistry (FFPE): 1:10-1:50
• Immunogen: Synthetic peptide corresponding to aa416-445 from the C-terminal region of human HRH3 conjugated to KLH (Q9Y5N1). Cellular Location: Cell Membrane
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-HH3R Pab (Rabbit ID 1071) in Jurkat cell line lysate (35ug/lane). HH3R(arrow) was detected using the purified Pab. This western blot identifies isoform two of HRH3. The accession number of HRH3 is CAC39434; Q9Y5N1.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with HRH3 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Related Product Information for anti-Hrh3 antibody

HRH3 is a presynaptic autoreceptor on histamine neurons in the brain and a presynaptic heteroreceptor in nonhistamine-containing neurons in both the CNS and PNS. The deduced 445aa HRH3 protein contains 7 predicted transmembrane domains. HRH3 has significant sequence homology to members of the biogenic amine subfamily of GPCRs, including an aspartic acid residue in the predicted third transmembrane domain, a hallmark of the biogenic amine receptor subfamily; this residue is the putative binding site for the primary amine. HRH3 shares 22% and 21.4% amino acid sequence homology with the H1 and H2 receptors, respectively. Expression of recombinant HRH3 in a variety of cell lines conferred an ability to inhibit adenylate cyclase in response to histamine, but not to acetylcholine or any other biogenic amine. Northern blot analysis of human tissues showed HRH3 expression only in the brain, with highest expression in the thalamus and caudate nucleus. RT-PCR showed expression in human small intestine, testis, and prostate. In situ hybridization of rat brain sections showed that HRH3 is abundantly expressed in brain. HRH3 was most notably observed throughout the thalamus, the ventromedial hypothalamus, and the caudate nucleus. Strong expression was also seen in layers II, V, and VIb of the cerebral cortex, in the pyramidal layers of the hippocampus, and in olfactory tubercle. In addition, HRH3 expression was found in the locus ceruleus and in the histaminergic cell bodies in the tuberomammillary nuclei.

Product Categories/Family for anti-Hrh3 antibody

Antibodies; Abs to G Protein Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 396080303
• NCBI GeneID: 85268
• NCBI Accession #: NP_001257499.1
• NCBI GenBank Nucleotide #: NM_001270570.1
• UniProt Accession #: Q9QYN8; Q9QYN6; Q9QYN7; Q9QYN9
• Molecular Weight: 48,588 Da
• NCBI Official Full Name: histamine H3 receptor isoform 8
• NCBI Official Synonym Full Names: histamine receptor H3
• NCBI Official Symbol: Hrh3
• NCBI Protein Information: histamine H3 receptor; H3R; HH3R
• UniProt Protein Name: Histamine H3 receptor
• Protein Family: Histamine H3 receptor
• UniProt Gene Name: Hrh3
• UniProt Synonym Gene Names: H3R; HH3R
• UniProt Entry Name: HRH3_RAT

NCBI Description

This gene encodes a histamine H3 receptor that belongs to the superfamily of G-protein coupled receptors. This protein functions as a presynaptic autoreceptor on histamine neurons in the brain, and a presynaptic heteroreceptor in nonhistamine-containing neurons in both the central and peripheral nervous systems. It is deemed a great target for the development of therapeutics for numerous disorders, including obesity, epilepsy, and such cognitive diseases as attention deficit hyperactivity disorder and Alzheimer's disease. Several alternatively spliced transcript variants encoding different isoforms, with different brain expression patterns and signaling properties, have been described for this gene. [provided by RefSeq, Jul 2012]

Uniprot Description

H3R: The H3 subclass of histamine receptors could mediate the histamine signals in CNS and peripheral nervous system. Signals through the inhibition of adenylate cyclase and displays high constitutive activity (spontaneous activity in the absence of agonist). Agonist stimulation of isoform 3 niether modified adenylate cyclase activity nor induced intracellular calcium mobilization. Belongs to the G-protein coupled receptor 1 family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass

Cellular Component: neuron projection; integral to membrane; plasma membrane; myelin sheath

Molecular Function: drug binding; histamine receptor activity

Biological Process: negative regulation of glutamate secretion; eating behavior; regulation of neurotransmitter levels; negative regulation of gamma-aminobutyric acid secretion; negative regulation of adenylate cyclase activity; drinking behavior; learning; regulation of norepinephrine secretion; response to organic cyclic substance; memory; G-protein coupled receptor protein signaling pathway; negative regulation of serotonin secretion; elevation of cytosolic calcium ion concentration; brain development; negative regulation of blood pressure; cognition; positive regulation of epithelial cell proliferation