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Western Blot (WB) (AMBP Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the AMBP antibody detected the AMBP protein (arrow).)

Rabbit anti-Human AMBP Polyclonal Antibody | anti-AMBP antibody

AMBP, NT (AMBP, HCP, ITIL, Protein AMBP, Alpha-1-microglobulin, Alpha-1 microglycoprotein, Complex-forming glycoprotein heterogeneous in charge, Inter-alpha-trypsin inhibitor light chain, Bikunin, EDC1, HI-30, Uronic-acid-rich protein, Trypstatin) (FITC)

Gene Names
AMBP; A1M; HCP; ITI; UTI; EDC1; HI30; ITIL; IATIL; ITILC
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
AMBP; Polyclonal Antibody; NT (AMBP; HCP; ITIL; Protein AMBP; Alpha-1-microglobulin; Alpha-1 microglycoprotein; Complex-forming glycoprotein heterogeneous in charge; Inter-alpha-trypsin inhibitor light chain; Bikunin; EDC1; HI-30; Uronic-acid-rich protein; Trypstatin) (FITC); Trypstatin; anti-AMBP antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-AMBP antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:10-50
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
AMBP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-104 amino acids from the N-terminal region of human AMBP.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(AMBP Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the AMBP antibody detected the AMBP protein (arrow).)

Western Blot (WB) (AMBP Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the AMBP antibody detected the AMBP protein (arrow).)

Immunohistochemistry (IHC)

(AMBP Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of AMBP Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (AMBP Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of AMBP Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Product Categories/Family for anti-AMBP antibody
References
Olsson, M.G., et al. Radiat. Res. 174(5):590-600(2010). Allhorn, M., et al. Blood 99(6):1894-1901(2002). Amoresano, A., et al. Eur. J. Biochem. 267(7):2105-2112(2000). Xu, Y., et al. J. Mol. Biol. 276(5):955-966(1998). Vetr, H., et al. Biol. Chem. Hoppe-Seyler 371(12):1185-1196(1990).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
259
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
protein AMBP preproprotein
NCBI Official Synonym Full Names
alpha-1-microglobulin/bikunin precursor
NCBI Official Symbol
AMBP
NCBI Official Synonym Symbols
A1M; HCP; ITI; UTI; EDC1; HI30; ITIL; IATIL; ITILC
NCBI Protein Information
protein AMBP; bikunin; complex-forming glycoprotein heterogeneous in charge; growth-inhibiting protein 19; inter-alpha-trypsin inhibitor light chain; protein HC; trypstatin; uristatin; uronic-acid-rich protein
UniProt Protein Name
Protein AMBP
Protein Family
UniProt Gene Name
AMBP
UniProt Synonym Gene Names
HCP; ITIL; Protein HC; ITI-LC
UniProt Entry Name
AMBP_HUMAN

NCBI Description

This gene encodes a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-1-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. This gene is located on chromosome 9 in a cluster of lipocalin genes. [provided by RefSeq, Jul 2008]

Uniprot Description

AMBP: Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 9q32-q33

Cellular Component: extracellular space; cell surface; intracellular membrane-bound organelle; plasma membrane; extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; protein homodimerization activity; calcium oxalate binding; IgA binding; heme binding; calcium channel inhibitor activity

Biological Process: negative regulation of JNK cascade; receptor-mediated endocytosis; viral reproduction; heme catabolic process; negative regulation of immune response; protein catabolic process; female pregnancy; cell adhesion; protein-chromophore linkage

Research Articles on AMBP

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Product Notes

The AMBP ambp (Catalog #AAA6273150) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AMBP, NT (AMBP, HCP, ITIL, Protein AMBP, Alpha-1-microglobulin, Alpha-1 microglycoprotein, Complex-forming glycoprotein heterogeneous in charge, Inter-alpha-trypsin inhibitor light chain, Bikunin, EDC1, HI-30, Uronic-acid-rich protein, Trypstatin) (FITC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AMBP can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:10-50 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the AMBP ambp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AMBP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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