Rabbit anti-Human AF4 Polyclonal Antibody | anti-AF4 antibody

AF4, NT (MLLT2, Mixed Lineage Leukemia, Translocated to, 2, ALL1 fused gene from chromosome 4, AF4, FEL) (MaxLight 550)

Cat. Num. AAA6272032

• Gene Names: AFF1; AF4; PBM1; MLLT2
• Reactivity: Human
• Applications: FLISA
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: AF4; Polyclonal Antibody; NT (MLLT2; Mixed Lineage Leukemia; Translocated to; 2; ALL1 fused gene from chromosome 4; FEL) (MaxLight 550); MLLT2; FEL; anti-AF4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-AF4 antibody: FLISA
• Application Notes: FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: AF4 (MLLT2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human MLLT2.
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Related Product Information for anti-AF4 antibody

MLLT2 is involved in acute leukemias through a chromosomal translocation t(4;11)(q21;q23) that involves mllt2 and mll/hrx. AF-4 (MLLT2), AF-9, and ENL proteins contain nuclear targeting sequences as well as serine-rich and proline-rich regions. Stretches abundant in basic amino acids are also present in the three proteins. These results suggest that the different proteins fused to ALL-1 polypeptide(s) provide similar functional domains. AF4 is a serine-and proline-rich putative transcription factor with a glutamine-rich carboxyl terminus. The composition of the complete MLL-AF4 fusion product argues that it may act through either a gain-of-function or a dominant negative mechanism in leukemogenesis.

Product Categories/Family for anti-AF4 antibody

Antibodies; Cancer Markers; Leukemia

References

Bursen, A., et al., Oncogene 23(37):6237-6249 (2004). Beausoleil, S.A., et al., Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135 (2004). Caslini, C., et al., Leukemia 18(6):1064-1071 (2004). Bertrand, F.E., et al., Leukemia 17(12):2454-2459 (2003). Reichel, M., et al., Leukemia 15(2):286-288 (2001).

NCBI and Uniprot Product Information

• NCBI GI #: 262359911
• NCBI GeneID: 4299
• NCBI Accession #: NP_001160165.1
• NCBI GenBank Nucleotide #: NM_001166693.1
• UniProt Accession #: P51825; B4DTU1; E9PBM3
• Molecular Weight: 131,422 Da
• NCBI Official Full Name: AF4/FMR2 family member 1 isoform 1
• NCBI Official Synonym Full Names: AF4/FMR2 family, member 1
• NCBI Official Symbol: AFF1
• NCBI Official Synonym Symbols: AF4; PBM1; MLLT2
• NCBI Protein Information: AF4/FMR2 family member 1; protein AF-4; proto-oncogene AF4; pre-B-cell monocytic leukemia partner 1; ALL1-fused gene from chromosome 4 protein; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 2
• UniProt Protein Name: AF4/FMR2 family member 1
• Protein Family: AF4/FMR2 family
• UniProt Gene Name: AFF1
• UniProt Synonym Gene Names: AF4; FEL; MLLT2; PBM1; Protein AF-4
• UniProt Entry Name: AFF1_HUMAN

Uniprot Description

AF-4: A chromosomal aberration involving AFF1 is associated with acute leukemias. Translocation t(4;11)(q21;q23) with MLL/HRX. The result is a rogue activator protein. Belongs to the AF4 family.

Protein type: Transcription factor; Oncoprotein

Chromosomal Location of Human Ortholog: 4q21

Cellular Component: transcription elongation factor complex

Molecular Function: protein binding; transcription factor activity

Biological Process: positive regulation of transcription, DNA-dependent