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Western Blot (WB) (ADH1C Antibody (Center) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the ADH1C antibody detected the ADH1C protein (arrow).)

Rabbit anti-Human ADH1C Polyclonal Antibody | anti-ADH1C antibody

ADH1C, ID (ADH1C, ADH3, Alcohol dehydrogenase 1C, Alcohol dehydrogenase subunit gamma) (Biotin)

Gene Names
ADH1C; ADH3
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
ADH1C; Polyclonal Antibody; ID (ADH1C; ADH3; Alcohol dehydrogenase 1C; Alcohol dehydrogenase subunit gamma) (Biotin); Alcohol dehydrogenase subunit gamma; anti-ADH1C antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-ADH1C antibody
Flow Cytometry (FC/FACS), ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
ELISA: 1:1,000
IF: 1:10-50
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
ADH1C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 238-268 amino acids from the Central region of human ADH1C.
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ADH1C Antibody (Center) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the ADH1C antibody detected the ADH1C protein (arrow).)

Western Blot (WB) (ADH1C Antibody (Center) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the ADH1C antibody detected the ADH1C protein (arrow).)

Western Blot (WB)

(Western blot analysis of ADH1C (arrow) using rabbit polyclonal ADH1C Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ADH1C gene.)

Western Blot (WB) (Western blot analysis of ADH1C (arrow) using rabbit polyclonal ADH1C Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ADH1C gene.)

Immunohistochemistry (IHC)

(ADH1C Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ADH1C Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (ADH1C Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ADH1C Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of ADH1C Antibody (Center) with T47D cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

Immunofluorescence (IF) (Confocal immunofluorescent analysis of ADH1C Antibody (Center) with T47D cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

Flow Cytometry (FC/FACS)

(ADH1C Antibody (Center) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (ADH1C Antibody (Center) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-ADH1C antibody
This gene encodes class I alcohol dehydrogenase, gamma subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class I alcohol dehydrogenase, consisting of several homo-and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster.
Product Categories/Family for anti-ADH1C antibody
References
Benzon Larsen, S., et al. Cancer Lett. (2010). In press: Sangrajrang, S., et al. Breast Cancer Res. Treat. (2010). Khan, A.J., et al. Drug Alcohol Depend (2010).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
126
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
39,868 Da
NCBI Official Full Name
alcohol dehydrogenase 1C
NCBI Official Synonym Full Names
alcohol dehydrogenase 1C (class I), gamma polypeptide
NCBI Official Symbol
ADH1C
NCBI Official Synonym Symbols
ADH3
NCBI Protein Information
alcohol dehydrogenase 1C; ADH, gamma subunit; alcohol dehydrogenase 3 (class I), gamma polypeptide; aldehyde reductase
UniProt Protein Name
Alcohol dehydrogenase 1C
Protein Family
UniProt Gene Name
ADH1C
UniProt Synonym Gene Names
ADH3
UniProt Entry Name
ADH1G_HUMAN

NCBI Description

This gene encodes class I alcohol dehydrogenase, gamma subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class I alcohol dehydrogenase, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster. [provided by RefSeq, Jul 2008]

Uniprot Description

ADH1C: class I alcohol dehydrogenase, gamma subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class I alcohol dehydrogenase, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster. [provided by RefSeq, Jul 2008]

Protein type: Xenobiotic Metabolism - metabolism by cytochrome P450; Amino Acid Metabolism - tyrosine; Oxidoreductase; EC 1.1.1.1; Mitochondrial; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Lipid Metabolism - fatty acid; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Cofactor and Vitamin Metabolism - retinol

Chromosomal Location of Human Ortholog: 4q23

Cellular Component: cytosol

Molecular Function: zinc ion binding; alcohol dehydrogenase activity

Biological Process: xenobiotic metabolic process; ethanol oxidation

Disease: Alcohol Dependence; Parkinson Disease, Late-onset

Research Articles on ADH1C

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Product Notes

The ADH1C adh1c (Catalog #AAA6271741) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ADH1C, ID (ADH1C, ADH3, Alcohol dehydrogenase 1C, Alcohol dehydrogenase subunit gamma) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ADH1C can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 ELISA: 1:1,000 IF: 1:10-50 IHC: 1:50-100 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ADH1C adh1c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ADH1C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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