Rabbit anti-Human ADD3 Polyclonal Antibody | anti-ADD3 antibody

ADD3, CT (ADD3, ADDL, Gamma-adducin, Adducin-like protein 70) (FITC)

Cat. Num. AAA6271720

• Gene Names: ADD3; ADDL
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ADD3; Polyclonal Antibody; CT (ADD3; ADDL; Gamma-adducin; Adducin-like protein 70) (FITC); Adducin-like protein 70; anti-ADD3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-ADD3 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ADD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 551-580 amino acids from the C-terminal region of human ADD3.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ADD3 Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the ADD3 antibody detected the ADD3 protein (arrow).)

Related Product Information for anti-ADD3 antibody

Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced adducin gamma transcripts encoding different isoforms have been described. The functions of the different isoforms are not known.

Product Categories/Family for anti-ADD3 antibody

Antibodies; Actin Related Proteins

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Garcia-Barcelo, M.M., et al. Hum. Mol. Genet. 19(14):2917-2925(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Seidlerova, J., et al. Am. J. Hypertens. 22(1):21-26(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 62912453
• NCBI GeneID: 120
• NCBI Accession #: NP_001112.2; NP_063968.1
• NCBI GenBank Nucleotide #: NM_001121.2
• UniProt Accession #: Q9UEY8; O43243; Q5VU09; Q92773; Q9UEY7; D3DRA8
• Molecular Weight: 79,155 Da
• NCBI Official Full Name: gamma-adducin isoform b
• NCBI Official Synonym Full Names: adducin 3 (gamma)
• NCBI Official Symbol: ADD3
• NCBI Official Synonym Symbols: ADDL
• NCBI Protein Information: gamma-adducin; adducin-like protein 70
• UniProt Protein Name: Gamma-adducin
• Protein Family: Gamma-adducin
• UniProt Gene Name: ADD3
• UniProt Synonym Gene Names: ADDL
• UniProt Entry Name: ADDG_HUMAN

NCBI Description

Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced adducin gamma transcripts encoding different isoforms have been described. The functions of the different isoforms are not known. [provided by RefSeq, Jul 2008]

Uniprot Description

ADD3: is a heteromeric protein composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. Adducins alpha and gamma are ubiquitously expressed, while the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions of alpha and beta have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced adducin gamma transcripts encoding different isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 10q25.2

Cellular Component: nucleoplasm; cytoskeleton; condensed nuclear chromosome; membrane; cytoplasm; plasma membrane; intercellular junction; cell cortex

Molecular Function: calmodulin binding; structural constituent of cytoskeleton; actin binding