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Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with MBS627106, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.)
Rabbit anti-Human, Mouse CBS Polyclonal Antibody
CBS, ID (Cystathionine beta-synthase, Serine Sulfhydrase, Beta-thionase)
Reactivity
Human, Mouse
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
CBS; Polyclonal Antibody; ID (Cystathionine beta-synthase; Serine Sulfhydrase; Beta-thionase); Anti -CBS; anti-CBS antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human CBS. Species Crossreactivity: mouse.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Applicable Applications for anti-CBS antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunogen
Synthetic peptide selected from the Center region of human CBS (KLH).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with MBS627106, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with MBS627106, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.)
Western Blot (WB)
(Western Blot analysis in Raji cell line, rat brain and cerebellum tissue lysates (35ug/lane) using MBS627106 . This demonstrates the CBS antibody detected the CBS protein (arrow).)
Western Blot (WB)
(Western Blot analysis in Raji cell line, rat brain and cerebellum tissue lysates (35ug/lane) using MBS627106 . This demonstrates the CBS antibody detected the CBS protein (arrow).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of 293 cell using MBS627106 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of 293 cell using MBS627106 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of 293 cells(bottom histogram) using MBS627106 compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of 293 cells(bottom histogram) using MBS627106 compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-CBS antibody
CBS acts as a homotetramer to catalyze the conversion of homocysteine to cystathionine, the first step in the transsulfuration pathway. This protein is allosterically activated by adenosyl-methionine and uses pyridoxal phosphate as a cofactor. Defects in this gene can cause cystathionine beta-synthase deficiency (CBSD), which can lead to homocystinuria.
Product Categories/Family for anti-CBS antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The CBS (Catalog #AAA627106) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CBS, ID (Cystathionine beta-synthase, Serine Sulfhydrase, Beta-thionase) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CBS can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 1:1,000 Western Blot: 1:100-1:500. Researchers should empirically determine the suitability of the CBS for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CBS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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