Rabbit anti-Human ACCN1 Polyclonal Antibody | anti-ACCN1 antibody

ACCN1, ID (ASIC2, ACCN, ACCN1, BNAC1, MDEG, Acid-sensing ion channel 2, Amiloride-sensitive brain sodium channel, Amiloride-sensitive cation channel 1, neuronal, Amiloride-sensitive cation channel neuronal 1, Brain sodium channel 1, Mammalian degenerin ho

Cat. Num. AAA6270837

• Gene Names: ASIC2; ACCN; BNC1; MDEG; ACCN1; BNaC1; ASIC2a; hBNaC1
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ACCN1; Polyclonal Antibody; ID (ASIC2; ACCN; BNAC1; MDEG; Acid-sensing ion channel 2; Amiloride-sensitive brain sodium channel; Amiloride-sensitive cation channel 1; neuronal; Amiloride-sensitive cation channel neuronal 1; Brain sodium channel 1; Mammalian degenerin ho; ASIC2; Mammalian degenerin homolog; anti-ACCN1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-ACCN1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: ACCN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 127~156 amino acids from the Center region of human ACCN1.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ACCN1 Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). ACCN1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with ACCN1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(ACCN1 Antibody (Center) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ACCN1 antibody

ACCN1 encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this protein may play a role in neurotransmission. In addition, a heteromeric association between this member and ACCN3 (variant 1) has been observed to co-assemble into proton-gated channels sensitive to gadolinium.

Product Categories/Family for anti-ACCN1 antibody

Antibodies; Ion Channel

References

Bashari, E., et.al., Am. J. Physiol., Cell Physiol. 296 (2), C372-C384 (2009). Chai, S., et.al., J. Biol. Chem. 282 (31), 22668-22677 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 9998944
• NCBI GeneID: 40
• NCBI Accession #: NP_001085.2
• NCBI GenBank Nucleotide #: NM_001094.4
• UniProt Accession #: Q16515; Q13553; Q6DJU1; Q8N3E2; E9PBX2
• Molecular Weight: 62,887 Da
• NCBI Official Full Name: acid-sensing ion channel 2 isoform MDEG1
• NCBI Official Synonym Full Names: acid sensing ion channel subunit 2
• NCBI Official Symbol: ASIC2
• NCBI Official Synonym Symbols: ACCN; BNC1; MDEG; ACCN1; BNaC1; ASIC2a; hBNaC1
• NCBI Protein Information: acid-sensing ion channel 2
• UniProt Protein Name: Acid-sensing ion channel 2
• UniProt Gene Name: ASIC2
• UniProt Synonym Gene Names: ACCN; ACCN1; BNAC1; MDEG; ASIC2; BNC1; BNaC1

NCBI Description

This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene may play a role in neurotransmission. In addition, a heteromeric association between this member and acid-sensing (proton-gated) ion channel 3 has been observed to co-assemble into proton-gated channels sensitive to gadolinium. Alternative splicing has been observed at this locus and two variants, encoding distinct isoforms, have been identified. [provided by RefSeq, Feb 2012]

Uniprot Description

ASIC2: Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Li(+) and K(+). Generates a biphasic current with a fast inactivating and a slow sustained phase. Heteromeric channel assembly seems to modulate. Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. ASIC2 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Channel, cation; Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, ion channel

Chromosomal Location of Human Ortholog: 17q11.2-q12

Cellular Component: plasma membrane

Molecular Function: amiloride-sensitive sodium channel activity; protein binding

Biological Process: central nervous system development; monovalent inorganic cation transport; peripheral nervous system development; regulation of blood coagulation; regulation of gene expression; sensory perception of sour taste; synaptic transmission