Rabbit anti-Human 14-3-3 beta, alpha Polyclonal Antibody | anti-YWHAB antibody

14-3-3 beta, alpha, CT (Protein 1054, Protein Kinase C Inhibitor Protein 1, KCIP-1, 14-3-3 Protein beta/alpha, N-terminally Processed, YWHAB) (APC)

Cat. Num. AAA6269303

• Gene Names: YWHAB; HS1; GW128; YWHAA; KCIP-1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: 14-3-3 beta; alpha; Polyclonal Antibody; CT (Protein 1054; Protein Kinase C Inhibitor Protein 1; KCIP-1; 14-3-3 Protein beta/alpha; N-terminally Processed; YWHAB) (APC); anti-YWHAB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human YWHAB.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-YWHAB antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human YWHAB (KLH).
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Product Categories/Family for anti-YWHAB antibody

Antibodies; 14-3-3

References

1. Komori, T., et al., Acta Neuropathol. 106(1):66-70 (2003). 2. Cavet, M.E., et al., J. Biol. Chem. 278(20):18376-18383 (2003). 3. Li, Y., et al., J. Biol. Chem. 278(16):13663-13671 (2003). 4. Shumway, S.D., et al., J. Biol. Chem. 278(4):2089-2092 (2003). 5. Parvaresch, S., et al., FEBS Lett. 532(3):357-362 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 4507949
• NCBI GeneID: 7529
• NCBI Accession #: NP_003395.1
• NCBI GenBank Nucleotide #: NM_003404.3
• UniProt Accession #: P31946; A8K9K2; E1P616
• Molecular Weight: 28,082 Da
• NCBI Official Full Name: 14-3-3 protein beta/alpha
• NCBI Official Synonym Full Names: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide
• NCBI Official Symbol: YWHAB
• NCBI Official Synonym Symbols: HS1; GW128; YWHAA; KCIP-1
• NCBI Protein Information: 14-3-3 protein beta/alpha; 14-3-3 alpha; protein 1054; brain protein 14-3-3, beta isoform; protein kinase C inhibitor protein 1; protein kinase C inhibitor protein-1; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alpha polypeptid
• UniProt Protein Name: 14-3-3 protein beta/alpha
• Protein Family: 14-3-3 protein
• UniProt Gene Name: YWHAB
• UniProt Synonym Gene Names: KCIP-1
• UniProt Entry Name: 1433B_HUMAN

NCBI Description

This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

14-3-3 beta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 20q13.1

Cellular Component: focal adhesion; cytoplasmic vesicle membrane; membrane; transcriptional repressor complex; perinuclear region of cytoplasm; cytoplasm; melanosome; nucleus; cytosol

Molecular Function: protein C-terminus binding; protein domain specific binding; protein binding; enzyme binding; histone deacetylase binding; protein complex binding; phosphoprotein binding; transcription corepressor activity; phosphoserine binding

Biological Process: epidermal growth factor receptor signaling pathway; negative regulation of protein amino acid dephosphorylation; positive regulation of catalytic activity; axon guidance; fibroblast growth factor receptor signaling pathway; activation of MAPKK activity; nerve growth factor receptor signaling pathway; apoptosis; protein heterooligomerization; MAPKKK cascade; small GTPase mediated signal transduction; Ras protein signal transduction; insulin receptor signaling pathway; cytoplasmic sequestering of protein; innate immune response; gene expression; vascular endothelial growth factor receptor signaling pathway; negative regulation of transcription, DNA-dependent; protein targeting