Rabbit anti-Human BRCA1, phosphorlated Polyclonal Antibody | anti-BRCA1 antibody

BRCA1, phosphorlated (Ser1423) (Breast Cancer Type 1 Susceptibility Protein, RING Finger Protein 53, RNF53)

Cat. Num. AAA626902

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: BRCA1; phosphorlated; Polyclonal Antibody; phosphorlated (Ser1423) (Breast Cancer Type 1 Susceptibility Protein; RING Finger Protein 53; RNF53); Anti -BRCA1; anti-BRCA1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human BRCA1.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-BRCA1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic phosphopeptide corresponding to human BRCA1 around the phosphorylation site of serine 1423 (H-G-SP-Q-P).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-BRCA1 antibody

BRCA1 contains at least two nuclear localization signals and is proposed to be a tumor suppressor protein. It is a serine phosphoprotein that undergoes hyper-phosphorylation during late G1-and S-phases of the cell cycle and is transiently dephosphorylated early after M-phase.1 BRCA1 protein alters in a qualitative and quantitative manner during cell cycle progression.1 The amount of BRCA1 protein is highest during S-phase and remains elevated toward G2/M, before it declines in early G1-phase.1 Inherited loss of BRCA1 function confers an increased susceptibility for both breast and ovarian cancer.

Product Categories/Family for anti-BRCA1 antibody

Antibodies; Abs to Transcription Factors; Zinc (Ring) Finger

NCBI and Uniprot Product Information

• NCBI GI #: 37953275
• NCBI GeneID: 449497
• UniProt Accession #: Q9GKK8; O46484
• Molecular Weight: 207,899 Da
• NCBI Official Full Name: BRCA1
• NCBI Official Symbol: BRCA1
• NCBI Protein Information: breast cancer type 1 susceptibility protein homolog
• UniProt Protein Name: Breast cancer type 1 susceptibility protein homolog
• Protein Family: Breast cancer type 1 susceptibility protein
• UniProt Gene Name: BRCA1
• UniProt Entry Name: BRCA1_PANTR

Uniprot Description

Function: E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. It is unclear whether it also mediates the formation of other types of polyubiquitin chains. The E3 ubiquitin-protein ligase activity is required for its tumor suppressor function. The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Regulates centrosomal microtubule nucleation. Required for normal cell cycle progression from G2 to mitosis. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation. Contributes to homologous recombination repair (HRR) via its direct interaction with PALB2, fine-tunes recombinational repair partly through its modulatory role in the PALB2-dependent loading of BRCA2-RAD51 repair machinery at DNA breaks

Subunit structure: Heterodimer with BARD1. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBN protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Component of the BRCA1-A complex, at least composed of the BRCA1, BARD1, UIMC1/RAP80, FAM175A/Abraxas, BRCC3/BRCC36, BRE/BRCC45 and BABAM1/NBA1. Interacts (via BRCT domains) with FAM175A/Abraxas and RBBP8. Associates with RNA polymerase II holoenzyme. Interacts with SMC1A and COBRA1/NELFB. Interacts (via BRCT domains) with phosphorylated BRIP1. Interacts with FANCD2 (ubiquitinated). Interacts with BAP1. Interacts with DCLRE1C/Artemis and CLSPN. Interacts with H2AFX (phosphorylated on 'Ser-140'). Interacts with CHEK1/CHK1. Interacts with BRCC3. Interacts (via BRCT domains) with ACACA (phosphorylated); the interaction prevents dephosphorylation of ACACA. Interacts with STK6/AURKA. Interacts with UBXN1. Part of a trimeric complex containing BRCA1, BRCA2 and PALB2. Interacts with PALB2 and this interaction is essential for its function in HRR. Interacts with BRCA2 only in the presence of PALB2 which serves as the bridging protein

Subcellular location: Nucleus

By similarity. Note: Localizes at sites of DNA damage at double-strand breaks (DSBs); recruitment to DNA damage sites is mediated by the BRCA1-A complex

Domain: The BRCT domains recognize and bind phosphorylated pSXXF motif on proteins. The interaction with the phosphorylated pSXXF motif of FAM175A/Abraxas, recruits BRCA1 at DNA damage sites

By similarity.The RING-type zinc finger domain interacts with BAP1

Post-translational modification: Phosphorylation at Ser-308 by STK6/AURKA is required for normal cell cycle progression from G2 to mitosis. Phosphorylated in response to IR, UV, and various stimuli that cause checkpoint activation, probably by ATM or ATR

By similarity.Autoubiquitinated, undergoes 'Lys-6'-linked polyubiquitination. 'Lys-6'-linked polyubiquitination does not promote degradation

Sequence similarities: Contains 2 BRCT domains.Contains 1 RING-type zinc finger.