Rabbit anti-Human, Mouse Hexokinase, Type IV Polyclonal Antibody

Hexokinase, Type IV, CT (HK IV, HKIV, Hexokinase-4, HK4, FGQTL3, Glucokinase, GCK, GK, GLK, Hexokinase-D, HHF3, HXKP, LGLK, MODY2)

Cat. Num. AAA626426

• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Hexokinase; Type IV; Polyclonal Antibody; CT (HK IV; HKIV; Hexokinase-4; HK4; FGQTL3; Glucokinase; GCK; GK; GLK; Hexokinase-D; HHF3; HXKP; LGLK; MODY2); Anti -Hexokinase; anti-Hexokinase; Type IV antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GCK. Species Crossreactivity: mouse.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-Hexokinase, Type IV antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic peptide selected from the C-terminal region of human GCK (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of Jurkat cell line lysates (35ug/lane) using MBS626426. GCK (arrow) was detected using MBS626426.)

Western Blot (WB)

(Western Blot analysis of GCK (arrow) using MBS626426. 293 cell lysates (2ug/lane) Lane 1: nontransfected Lane 2: transiently transfected with the GCK gene.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue using MBS626426, followed by peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of MBS626426 for immunohistochemistry. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-Hexokinase, Type IV antibody

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. Alternative splicing of the gene for GCK results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in the gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), also called maturity onset diabetes of the young, type 2 (MODY2); mutations have also been associated with persistent hyperinsulinemic hypoglycemia of infancy (PHHI).

Product Categories/Family for anti-Hexokinase, Type IV antibody

Antibodies; Abs to Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 160321
• NCBI Official Full Name: hexokinase type IV