Immunohistochemistry (IHC)
(Immunohistochemistry of a mouse lymph node cryosection with C2255-05B followed by IgG (HRP) Goat anti Rat IgG. Low power)
Rat anti-Human CD4 Monoclonal Antibody | anti-CD4 antibody
CD4 (CD4 Receptor, CD4mut, T Cell Surface Antigen T4/Leu3, T Cell Surface Glycoprotein CD4, L3T4, LY-4, Tac antigen, p55, Interleukin 2 Receptor alpha, IL2Ra, T Cell Growth Factor Receptor) (MaxLight 550)
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified by Protein G Affinity Chromatography
Synonyms
CD4; Monoclonal Antibody; CD4 (CD4 Receptor; CD4mut; T Cell Surface Antigen T4/Leu3; T Cell Surface Glycoprotein CD4; L3T4; LY-4; Tac antigen; p55; Interleukin 2 Receptor alpha; IL2Ra; T Cell Growth Factor Receptor) (MaxLight 550); L3T4 ANTIGEN; anti-CD4 antibody
Host
Rat
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
10A3
Specificity
Recognizes mouse CD4 at 55kD.
Purity/Purification
Purified by Protein G Affinity Chromatography
Form/Format
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight550.
Applicable Applications for anti-CD4 antibody
Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
Immunogen
Mouse CD4
Conjugate
MaxLight550
Preparation and Storage
Store at 4 degree C. Do Not Freeze.
Immunohistochemistry (IHC)
(Immunohistochemistry of a mouse lymph node cryosection with C2255-05B followed by IgG (HRP) Goat anti Rat IgG. Low power)
Immunohistochemistry (IHC)
(Immunohistochemistry of a mouse lymph node cryosection with C2255-05B followed by IgG (HRP) Goat anti Rat IgG. Low power)
Immunohistochemistry (IHC)
(Immunohistochemistry of a mouse lymph node cryosection with C2255-05B followed by IgG (HRP) Goat anti Rat IgG. Medium power)
Immunohistochemistry (IHC)
(Immunohistochemistry of a mouse lymph node cryosection with C2255-05B followed by IgG (HRP) Goat anti Rat IgG. Medium power)
Flow Cytometry (FC/FACS)
(Figure A: Flow Cytometry on murine splenocytes using C2255-05B. C2253-38F CD3 (APC) and MBS655606 IgG2b Negative Isotype Control (PE) Figure B: Flow Cytometry on murine splenocytes using C2255-05B. C2253-38F CD3 (APC) and C2255-04M CD4 (PE) )
Flow Cytometry (FC/FACS)
(Figure A: Flow Cytometry on murine splenocytes using C2255-05B. C2253-38F CD3 (APC) and MBS655606 IgG2b Negative Isotype Control (PE) Figure B: Flow Cytometry on murine splenocytes using C2255-05B. C2253-38F CD3 (APC) and C2255-04M CD4 (PE) )
Product Categories/Family for anti-CD4 antibody
References
1. Dialynas, D.P. et al. (1983) Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule. J Immunol.131:2445-2451 2. Wilde, D.B. et al. (1983) Evidence implicating L3T4 in class II MHC antigen reactivity; monoclonal antibody GK1.5 (anti-L3T4a) blocks class II MHC antigen-specific proliferation, release of lymphokines, and binding by cloned murine helper T lymphocyte lines. J Immunol. 131:2178-2183. 3. Dialynas, D.P. et al. (1983) Characterization of the murine antigenic determinant, designated L3T4a, recognized by monoclonal antibody GK1.5: expression of L3T4a by functional T cell clones appears to correlate primarily with class II MHC antigen-reactivity. Immunol Rev. 74:29-56.
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Product Notes
The CD4 (Catalog #AAA6254602) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The CD4 (CD4 Receptor, CD4mut, T Cell Surface Antigen T4/Leu3, T Cell Surface Glycoprotein CD4, L3T4, LY-4, Tac antigen, p55, Interleukin 2 Receptor alpha, IL2Ra, T Cell Growth Factor Receptor) (MaxLight 550) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD4 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB). Researchers should empirically determine the suitability of the CD4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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