Rabbit anti-Mouse Trefoil Factor 1 Polyclonal Antibody

Trefoil Factor 1 (Tff1, Bcei, Ps2, Protein pS2)

Cat. Num. AAA625092

• Reactivity: Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Trefoil Factor 1; Polyclonal Antibody; Trefoil Factor 1 (Tff1; Bcei; Ps2; Protein pS2); Anti -Trefoil Factor 1 (Tff1; anti-Trefoil Factor 1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse Trefoil Factor 1. Epitope: ~C-terminal
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, 0.1% BSA. Reconstitute in 100ul of sterile PBS.

• Applicable Applications for anti-Trefoil Factor 1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot and ELISA.; Dilution: Western Blot: 1-10ug/ml (Chemiluminescence); ELISA: 1:100,000; 50-100ng of T8269-73A control peptide/well
• Immunogen: Synthetic peptide corresponding to the C-terminal (15aa) of mouse TFF1 (Accession #Q08423), (KLH). Species sequence homology: rat 78%, human 0%.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Trefoil Factor 1 antibody

Minor disruption of the GI surface layer of the cells occurs frequently, such that maintaining epithelial integrity is of crucial importance. Normal epithelial repair requires restitution and regeneration. During restitution, within minutes after injury, epithelial cells spread and migrate across the basement membrane to re-establish surface cell continuity. Trefoil factor family (TFF) proteins play an important role in restitution by influencing the migration of cells without promotion of proliferation or tumorigenesis. Regeneration occurs later on and involves proliferation and differentiation of epithelial cells and restoration of specialized elements. Three mouse TFFs have been identified to date: TFF1 (formerly pS2) and TFF3 (formerly hITF), with one trefoil domain each, and TFF2 (formerly hSP), with two trefoil domains. TFF1: rat: 81aa; human: 84aa; mouse: 87aa - 9.67kD; Mouse chromosome: 17A3.3. Expressed in a number of carcinomas including breast (50%), pancreas (70-75%), stomach (50-55%), and large bowel (58%).

Product Categories/Family for anti-Trefoil Factor 1 antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 10280534
• NCBI Official Full Name: trefoil factor 1
• Protein Family: Trefoil factor

NCBI Description

Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. This gene, which is expressed in the gastric mucosa, has also been studied because of its expression in human tumors. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008]

Uniprot Description

TFF1: Stabilizer of the mucous gel overlying the gastrointestinal mucosa that provides a physical barrier against various noxious agents. May inhibit the growth of calcium oxalate crystals in urine.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: extracellular space

Molecular Function: protein binding; growth factor activity

Biological Process: response to peptide hormone stimulus; negative regulation of cell proliferation; maintenance of gastrointestinal epithelium; carbohydrate metabolic process; digestion; cell differentiation; response to iron ion; response to estradiol stimulus