Rabbit anti-Mouse BLNK Polyclonal Antibody | anti-BLNK antibody

BLNK (B cell Linker, BASH, Bca, B-cell Adapter Containing a SH2 Domain Protein, B-cell Adapter Containing a Src Homology 2 Domain Protein, Cytoplasmic Adapter Protein, Lymphocyte Antigen 57, Ly57, Ly-57, Lyw-57, Src Homology 2 Domain-containing Leukocyte

Cat. Num. AAA624966

• Gene Names: BLNK; AGM4; BASH; LY57; SLP65; BLNK-S; SLP-65; MGC111051
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Purified; Purified
• Synonyms: BLNK; Polyclonal Antibody; BLNK (B cell Linker; BASH; Bca; B-cell Adapter Containing a SH2 Domain Protein; B-cell Adapter Containing a Src Homology 2 Domain Protein; Cytoplasmic Adapter Protein; Lymphocyte Antigen 57; Ly57; Ly-57; Lyw-57; Src Homology 2 Domain-containing Leukocyte; Anti -BLNK (B cell Linker; anti-BLNK antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse BLNK.
• Purity/Purification: Purified; Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.

• Applicable Applications for anti-BLNK antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.
• Immunogen: Fusion protein corresponding to aa71-356 of human BLNK.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-BLNK antibody

BLNK (B cell linker protein) is an adaptor molecule that plays key role in B cell activation initiated by cross-linking the B cell receptor (BCR). This activation leads to an increase in the tyrosine phosphorylation of a number of proteins due to activation of nonreceptor protein tyrosine kinases (PTK) including Syk, which is responsible for the tyrosine phosphorylation of BLNK. BLNK is cytosolic protein identified as two phosphoproteins migrating at 68 and 70kD in SDS/PAGE (alternatively spliced forms of human BLNK). Mapping of the tyrosine residues in BLNK reveal three tyrosines that bind PLC-gamma2, and two tyrosines that bind the Tec family kinase Btk, the Vav nucleotide exchange factor and the adaptor Nck.

Product Categories/Family for anti-BLNK antibody

Antibodies; Abs to B Cells

NCBI and Uniprot Product Information

• NCBI GI #: 166235901
• NCBI GeneID: 29760
• NCBI Accession #: NP_001107566.1
• NCBI GenBank Nucleotide #: NM_001114094.1
• UniProt Accession #: Q2MD52; Q2MD40; Q2MD42; Q2MD44; Q2MD46; Q2MD47; Q2MD48; Q2MD49; Q2MD50; Q2MD51
• Molecular Weight: 48,201 Da
• NCBI Official Full Name: B-cell linker protein isoform 2
• NCBI Official Synonym Full Names: B-cell linker
• NCBI Official Symbol: BLNK
• NCBI Official Synonym Symbols: AGM4; BASH; LY57; SLP65; BLNK-S; SLP-65; MGC111051
• NCBI Protein Information: B-cell linker protein; OTTHUMP00000020167; OTTHUMP00000020168; B cell linker protein; cytoplasmic adapter protein; B-cell adapter containing a SH2 domain protein; B-cell adapter containing a Src homology 2 domain protein; Src homology 2 domain-containing leukocyte protein of 65 kDa
• UniProt Protein Name: B-cell linker protein
• Protein Family: B-cell linker protein
• UniProt Gene Name: BASH
• UniProt Synonym Gene Names: BLNK
• UniProt Entry Name: Q2MD52_HUMAN

NCBI Description

This gene encodes a cytoplasmic linker or adaptor protein that plays a critical role in B cell development. This protein bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. The phosphorylation of five tyrosine residues is necessary for this protein to nucleate distinct signaling effectors following B cell receptor activation. Mutations in this gene cause hypoglobulinemia and absent B cells, a disease in which the pro- to pre-B-cell transition is developmentally blocked. Deficiency in this protein has also been shown in some cases of pre-B acute lymphoblastic leukemia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Uniprot Description

BLNK iso3: an adaptor protein that bridges the B-cell receptor-associated kinases (BCR) with a multitude of signaling pathways, regulating biologic outcomes of B-cell function and development. Plays an important role in BCR-mediated PLCG1 activation and Ca(2 ) mobilization. Does not seem to be not required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition. Following BCR activation, phosphorylated on tyrosine residues by SYK and LYN. When phosphorylated, serves as a scaffold to assemble downstream targets of antigen activation, including PLCG1, VAV1, GRB2 and NCK1. Its phosphorylation is required for both Ca(2 ) and MAPK signaling pathways. Defects in BLNK are the cause of hypoglobulinemia and absent B-cells.It has tumor supressor activity that is lost in many cases of childhood pre-B acute lymphoblastic leukemia (ALL). Two alternatively spliced isoforms have been described; these are differentially involved in activation and apoptosis of B lymphocytes.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 10q23.2-q23.33

Cellular Component: plasma membrane; intracellular; cytosol

Molecular Function: protein binding; SH3/SH2 adaptor activity; transmembrane receptor protein tyrosine kinase adaptor protein activity

Biological Process: positive regulation of signal transduction; B cell differentiation; inflammatory response; transmembrane receptor protein tyrosine kinase signaling pathway; humoral immune response